Utility and limitations of T. gondii-specific IgM serum antibodies in the diagnosis of congenital toxoplasmosis in Porto Alegre

McLeod, Rima

doi:10.1016/j.jped.2014.04.001

Cited by 7 publications

(9 citation statements)

References 15 publications

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“…The important work from France [ 46 •, 51 •, 52 •, 56 ••, 60 ••, 62 ••, 66 ••, 67 ••] and Austria [ 53 ••] with some data from the USA [ 1 ••, 2 , 3 ••, 4 ••, 5 ••, 6 ••, 7 ••, 8 ••, 9 ••, 10 ••, 11 ••, 12 , 13 , 14 ••, 15 ••, 16 ••, 17 ••, 18 •, 19 •, 20 •, 21 •, 22 •, 23 , 24 ••, 25 , 26 , 27 •, 28 •, 29 , 30 , 31 •, 32 •, 33 ••, 34 •, 35 •, 36 , 37 •, 38 •, 39 ••, 40 •, 41 •, 42 ••, 43 ••, 44 ••, 45 , 46 •, 47 ••, 48 ••, 49 , 50 •, 51 •, 52 •, 53 ••, 54 , 55 ••, 56 ••, 57 •, 58 ••, 59 •] has demonstrated that congenital toxoplasmosis is a treatable and...…”

Section: Review Of Foundational Studies and Updates Concerning Clinic...mentioning

confidence: 99%

“…Methodology has been presented in ongoing reports throughout that time. Methodology has included obtaining information about this infection, its epidemiology, diagnosis, treatment and outcomes (Tables 1 , 2 and 3 ) [ 1 ••, 2 , 3 ••, 4 ••, 5 ••, 6 ••, 7 ••, 8 ••, 9 ••, 10 ••, 11 ••, 12 , 13 , 14 ••, 15 ••, 16 ••, 17 ••, 18 •, 19 •, 20 •, 21 •, 22 •, 23 , 24 ••, 25 , 26 , 27 •, 28 •, 29 , 30 , 31 •, 32 •, 33 ••, 34 •, 35 •, 36 , 37 •, 38 •, 39 ••, 40 •, 41 •, 42 ••, 43 ••, 44 ••, 45 , 46 •, 47 ••, 48 ••, 49 , 50 •, 51 •, 52 •, 53 ••, 54 , 55 ••, 56 ••, 57 •, 58 ••, 59 •]. Results are compared with earlier studies [ 70 , 71 ••].…”

Section: Review Of Foundational Studies and Updates Concerning Clinic...mentioning

confidence: 99%

“…Herein, we present an introduction to, and an overview and review of work [ 1 ••, 2 , 3 ••, 4 ••, 5 ••, 6 ••, 7 ••, 8 ••, 9 ••, 10 ••, 11 ••, 12 , 13 , 14 ••, 15 ••, 16 ••, 17 ••, 18 •, 19 •, 20 •, 21 •, 22 •, 23 , 24 ••, 25 , 26 , 27 •, 28 •, 29 , 30 , 31 •, 32 •, 33 ••, 34 •, 35 •, 36 , 37 •, 38 •, 39 ••, 40 •, 41 •, 42 ••, 43 ••, 44 ••, 45 , 46 •, 47 ••, 48 ••, 49 , 50 •, 51 •, 52 •, 53 ••, 54 , 55 ••, 56 ••, 57 •, 58 ••, 59 •, 60 ••, 61 , 62 ••, 63 •, 64 ••, 65 , 66 ••, 67 ••, 68 ••, 69 , 70 , 71 ••, 72 ••, 73 •...…”

Section: Introductionmentioning

confidence: 99%

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Building Programs to Eradicate Toxoplasmosis Part I: Introduction and Overview

Felín¹,

Wang

Moreira

et al. 2022

Curr Pediatr Rep

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Purpose of Review Review building of programs to eliminate Toxoplasma infections. Recent Findings Morbidity and mortality from toxoplasmosis led to programs in USA, Panama, and Colombia to facilitate understanding, treatment, prevention, and regional resources, incorporating student work. Summary Studies foundational for building recent, regional approaches/programs are reviewed. Introduction provides an overview/review of programs in Panamá, the United States, and other countries. High prevalence/risk of exposure led to laws mandating testing in gestation, reporting, and development of broad-based teaching materials about Toxoplasma. These were tested for efficacy as learning tools for high-school students, pregnant women, medical students, physicians, scientists, public health officials and general public. Digitized, free, smart phone application effectively taught pregnant women about toxoplasmosis prevention. Perinatal infection care programs, identifying true regional risk factors, and point-of-care gestational screening facilitate prevention and care. When implemented fully across all demographics, such programs present opportunities to save lives, sight, and cognition with considerable spillover benefits for individuals and societies.

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Section: Review Of Foundational Studies and Updates Concerning Clinic...mentioning

confidence: 99%

Section: Review Of Foundational Studies and Updates Concerning Clinic...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Building Programs to Eradicate Toxoplasmosis Part I: Introduction and Overview

Felín¹,

Wang

Moreira

et al. 2022

Curr Pediatr Rep

Self Cite

View full text Add to dashboard Cite

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“…[1524] Negative results of IgM in the diagnosis of neonates born from mothers with acute toxoplasmosis can be related to perform an antibody test at a time near the onset of infection in which case the IgM has been either not yet produced or is not detectable. [25] According to the WHO criteria, preterm birth is gestational age <37 weeks and low birth weight is the birth weight ≤2499 g.[26] In this study, a case of low birth weight was seen, but no premature birth was observed. Some studies have not reported a significant relationship between low birth weight and prematurity with anti- T. gondii maternal serum profiles.…”

Section: Discussionmentioning

confidence: 68%

Evaluation of Toxoplasma gondii B1 gene in Placental Tissues of Pregnant Women with Acute Toxoplasmosis

et al. 2018

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Background:One of the consequences of toxoplasmosis is the risk of passing it from mother to fetus and the onset of congenital toxoplasmosis during pregnancy. The purpose of this study was to evaluate the B1 gene of Toxoplasma gondii in the placental tissues of pregnant women with acute toxoplasmosis.Materials and Methods:The study was a cross-sectional study. Serum samples of pregnant women who attended to Fatemieh Hospital of Hamadan University of Medical Sciences were tested for immunoglobulin G (IgG) antibodies against T. gondii by enzyme-linked immunosorbent assay. Then, polymerase chain reaction was used to identify the specific B1 gene of T. gondii in IgG seropositive women. The placental tissues of the pregnant women with positive serum B1 gene examined for this gene. Anti-Toxoplasma immunoglobulin M (IgM) was performed on the umbilical cord and neonate blood.Results:Anti-Toxoplasma IgG was detected in 167 out of 653 (25.6%) pregnant women. T. gondii B1 gene was identified in 36 out of 167 (21.6%) of IgG seropositive women. After delivery, the B1 gene was evaluated in 15 out of 36 (41.7%) patients’ placental tissues, 13 of which were positive for this gene (86.7%). Anti-Toxoplasma IgM was detected neither in any umbilical cord nor in neonatal blood samples. All newborns, with the exception of one case, were born with normal birth weight and in term birth.Conclusion:The B1 gene was detected in 86.7% of the placental tissue of women who were involved in acute toxoplasmosis during pregnancy.

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“…Detection of T. gondii DNA by molecular methods is quite controversial as there are several techniques and markers to identify the DNA of the parasite, and there is no consensus on which protocol is the best. Moreover, the treatment of pregnant women can modify the results of serological and molecular tests, as has been described by some authors (Lago et al, 2014;Lefevre-Pettazzoni et al, 2007;McLeod, 2014;Okay et al, 2009;Robert-Gangneux and Dardé, 2012).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazil

Murata

Ferreira

Pereira-Chioccola

et al. 2017

Diagnostic Microbiology and Infectious Disease

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Toxoplasmosis during pregnancy can have severe consequences. The use of sensitive and specific serological and molecular methods is extremely important for the correct diagnosis of the disease. We compared the ELISA and ELFA serological methods, conventional PCR (cPCR), Nested PCR and quantitative PCR (qPCR) in the diagnosis of Toxoplasma gondii infection in pregnant women without clinical suspicion of toxoplasmosis (G1=94) and with clinical suspicion of toxoplasmosis (G2=53). The results were compared using the Kappa index, and the sensitivity, specificity, positive predictive value and negative predictive value were calculated. The results of the serological methods showed concordance between the ELISA and ELFA methods even though ELFA identified more positive cases than ELISA. Molecular methods were discrepant with cPCR using B22/23 primers having greater sensitivity and lower specificity compared to the other molecular methods.

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Utility and limitations of T. gondii-specific IgM serum antibodies in the diagnosis of congenital toxoplasmosis in Porto Alegre

Cited by 7 publications

References 15 publications

Building Programs to Eradicate Toxoplasmosis Part I: Introduction and Overview

Building Programs to Eradicate Toxoplasmosis Part I: Introduction and Overview

Evaluation of Toxoplasma gondii B1 gene in Placental Tissues of Pregnant Women with Acute Toxoplasmosis

Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazil

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