Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazil

Murata, Fernando Henrique Antunes; Ferreira, Marina Neves; Pereira-Chioccola, Vera Lúcia; Spegiorin, Lígia Cosentino Junqueira Franco; Meira-Strejevitch, Cristina da Silva; Gava, Ricardo; Silveira-Carvalho, Aparecida Perpétuo; Mattos, Luiz Carlos de; Mattos, Cinara Cássia Brandão de

doi:10.1016/j.diagmicrobio.2017.06.004

Cited by 8 publications

(8 citation statements)

References 66 publications

(70 reference statements)

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“…Routine tests for the diagnosis of T. gondii infection are based on serology [ 15 ]. Enzyme-linked immunosorbent assays and enzyme-linked fluorescent assays are tests currently used for detection of anti- T. gondii IgG and IgM antibodies in humans [ 16 ]. T. gondii commercial IgM diagnostic test kits can yield a number of false-positive results [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Knowledge and Practices of Toxoplasmosis among Clinical Laboratory Professionals: A Cross-Sectional Study in Durango, Mexico

Alvarado‐Esquivel

Sánchez‐Anguiano²,

Berumen-Segovia

et al. 2017

IJERPH

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Background: The aim of this study was to determine the level of knowledge and practices about toxoplasmosis in a sample of clinical laboratory professionals in Mexico. Methods: 192 clinical laboratory professionals were surveyed. They were asked about (1) Toxoplasma gondii; (2) clinical manifestations, diagnosis, treatment, and epidemiology of toxoplasmosis; and (3) their practices with respect to toxoplasmosis. Results: The range of animals infected by T. gondii was known by 44.8% of participants. Clinical aspects of toxoplasmosis were known by up to 44.3% of subjects. Correct answers about the interpretation of serological markers of T. gondii infection were provided by up to 32.8% of participants. A minority (32.2%) of participants knew about a high number of false positive results of anti-T. gondii IgM antibody tests. Most participants (90.1%) did not know what the anti-T. gondii IgG avidity test was. Up to 55.7% of participants provided incorrect answers about the interpretation of serology tests for the treatment of pregnant women. Common routes of T. gondii infection were known by <15% of participants. Most (84.4%) participants had not performed tests for detection T. gondii infection. Conclusions: Results indicate incomplete knowledge of T. gondii infection and toxoplasmosis and a limited practice of laboratory tests among the professionals surveyed.

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Section: Introductionmentioning

confidence: 99%

Knowledge and Practices of Toxoplasmosis among Clinical Laboratory Professionals: A Cross-Sectional Study in Durango, Mexico

Alvarado‐Esquivel

Sánchez‐Anguiano²,

Berumen-Segovia

et al. 2017

IJERPH

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“…The sensitivity of B1 gene was higher when samples were submitted just to one-round PCR using B22/23 primer than compared to one-round PCR using JW62/63 and nested-PCR. Primer B22/23 amplifies a 115-base pair sequence of B1 gene and has been reported as highly sensitive and specific primer used to detect T. gondii DNA in blood, cerebrospinal fluid, and amniotic fluid (Vidal et al, 2004;Okay et al, 2009;Mattos et al, 2011;Camilo et al, 2017;Murata et al, 2017). In a study conducted by Camilo et al (2017) evaluating two real time-PCR for B1 gene and REP-529 with a conventional PCR using the B22/23 primer, the authors found that REP-529 had better performance compared with the B1 gene.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Serological and Molecular Tests Used for the Identification of Toxoplasma gondii Infection in Patients Treated in an Ophthalmology Clinic of a Public Health Service in São Paulo State, Brazil

Murata

Previato

Frederico

et al. 2020

Front. Cell. Infect. Microbiol.

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Ocular toxoplasmosis is one of the most common complications caused by the infection with the parasite Toxoplasma gondii. The risk of developing eye lesions and impaired vision is considered higher in Brazil than other countries. The clinical diagnosis is difficult and the use of sensitive and specific laboratorial methods can aid to the correct diagnosis of this infection. We compared serological methods ELISA and ELFA, and molecular cPCR, Nested PCR and qPCR for the diagnosis of T. gondii infection in groups of patients clinically evaluated with ocular diseases non-toxoplasma related (G1 = 185) and with lesions caused by toxoplasmosis (G2 = 164) in an Ophthalmology clinic in Brazil. Results were compared by the Kappa index, and sensitivity (S), specificity (E), positive predictive value (PPV), and negative (NPV) were calculated. Serologic methods were in agreement with ELISA more sensitive and ELFA more specific to characterize the acute and chronic infections while molecular methods were discrepant where qPCR presented higher sensitivity, however, lower specificity when compared to cPCR and Nested PCR.

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“…O método de diagnóstico utilizado em ambos estudos é a técnica de ELISA. Em um estudo realizado em São Paulo comparando testes sorológicos que são amplamente utilizados no diagnóstico de toxoplasmose gestacional por apresentarem alta sensibilidade e especificidade, não foi encontrada diferença estatística entre métodos sorológicos (Murata et al, 2017).…”

Section: Discussionunclassified

Soroprevalência e fatores de risco para toxoplasmose em gestantes na região metropolitana de Goiânia, Goiás, Brasil

Silveira¹,

Filho

Oliveira

et al. 2020

BJHR

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A toxoplasmosis is a worldwide zoonosis caused by the protozoan Toxoplasma gondii. The greatest relevance of toxoplasmosis occurs when an infection develops in the gestational period, as vertical transmission may occur. The aim was to analyze the seroprevalence of toxoplasmosis in 1,007 blood samples and risk factors associated with seropositivity in the city of Goiânia and metropolitan region. We collected peripheral blood samples from 1.007 pregnant women at metropolitan area of Goiânia, from 2014 to 2016. All pregnant women who agreed to participate, signed the informed consent form and answered the socioeconomic questionnaire.

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Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazil

Cited by 8 publications

References 66 publications

Knowledge and Practices of Toxoplasmosis among Clinical Laboratory Professionals: A Cross-Sectional Study in Durango, Mexico

Knowledge and Practices of Toxoplasmosis among Clinical Laboratory Professionals: A Cross-Sectional Study in Durango, Mexico

Evaluation of Serological and Molecular Tests Used for the Identification of Toxoplasma gondii Infection in Patients Treated in an Ophthalmology Clinic of a Public Health Service in São Paulo State, Brazil

Soroprevalência e fatores de risco para toxoplasmose em gestantes na região metropolitana de Goiânia, Goiás, Brasil

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