Evaluation of Toxoplasma gondii B1 gene in Placental Tissues of Pregnant Women with Acute Toxoplasmosis

Abstract

Background:One of the consequences of toxoplasmosis is the risk of passing it from mother to fetus and the onset of congenital toxoplasmosis during pregnancy. The purpose of this study was to evaluate the B1 gene of Toxoplasma gondii in the placental tissues of pregnant women with acute toxoplasmosis.Materials and Methods:The study was a cross-sectional study. Serum samples of pregnant women who attended to Fatemieh Hospital of Hamadan University of Medical Sciences were tested for immunoglobulin G (IgG) antibodies against T. gondii by enzyme-linked immunosorbent assay. Then, polymerase chain reaction was used to identify the specific B1 gene of T. gondii in IgG seropositive women. The placental tissues of the pregnant women with positive serum B1 gene examined for this gene. Anti-Toxoplasma immunoglobulin M (IgM) was performed on the umbilical cord and neonate blood.Results:Anti-Toxoplasma IgG was detected in 167 out of 653 (25.6%) pregnant women. T. gondii B1 gene was identified in 36 out of 167 (21.6%) of IgG seropositive women. After delivery, the B1 gene was evaluated in 15 out of 36 (41.7%) patients’ placental tissues, 13 of which were positive for this gene (86.7%). Anti-Toxoplasma IgM was detected neither in any umbilical cord nor in neonatal blood samples. All newborns, with the exception of one case, were born with normal birth weight and in term birth.Conclusion:The B1 gene was detected in 86.7% of the placental tissue of women who were involved in acute toxoplasmosis during pregnancy.

Key biomarkers in cerebral arteriovenous malformations: Updated review

“…The transcription factor NF-κB controls a number of genes involved in inflammation. 10,14,78 In comparison with pNF-κB, Aziz et al 58 found that ECs and perivascular infiltrating inflammatory cells had greater amounts of NF-κB and IB. pNF-κB was mostly found in the nuclei of invading inflammatory cells and ECs.…”

“…In both untreated and treated CAVM patients, the AG genotype of the TNF‐α‐238 G>A SNP was linked to a greater risk of ICH. The transcription factor NF‐κB controls a number of genes involved in inflammation 10,14,78 . In comparison with pNF‐κB, Aziz et al 58 found that ECs and perivascular infiltrating inflammatory cells had greater amounts of NF‐κB and IB.…”

“…[9][10][11] It is the process in which the immune system detects and eliminates harmful and external stimuli and starts the healing process. [12][13][14] Generally, the extent and effects of chronic inflammation vary with the cause of the injury and the ability of the body to repair and overcome damage. 15 Inflammatory and biochemical mediators cause mitochondrial dysfunction and oxidative stress, such as enhanced release of free radical molecules, uric acid crystals, advanced glycation end products, homocysteine, and oxidized lipoproteins.…”

“…Inflammation is part of the body's defense mechanism 9–11 . It is the process in which the immune system detects and eliminates harmful and external stimuli and starts the healing process 12–14 . Generally, the extent and effects of chronic inflammation vary with the cause of the injury and the ability of the body to repair and overcome damage 15 .…”

Toxoplasma gondii molecular detection and phylogenetic analysis in hemodialysis patients from Khuzestan, Southwest Iran.

“…Both injecting sensitive laboratory animals with the parasites and cultivating them on living tissue are labor-intensive and insensitive ways for nding parasites in the blood. As a result, today's parasite detection in biological products uses the highly sensitive and speci c PCR approach [17]. This study's objectives are to identify the T. gondii parasite using Nested-PCR tests in the blood of individuals with chronic renal failure and to assess their genetic diversity through sequencing.…”