Cissampelos sympodialis has anti-viral effect inhibiting dengue non-structural viral protein-1 and pro-inflammatory mediators

Leite, Fagner Carvalho; Mello, Cíntia da Silva; Fialho, Luciana Luciana; Marinho, Cíntia Ferreira; Lima, Ana Luisa de Araujo; Barbosa-Filho, José Maria; Kubelka, Claire Fernandes; Piuvezam, Márcia Regina

doi:10.1016/j.bjp.2016.03.013

Cited by 7 publications

(4 citation statements)

References 20 publications

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“… Lima et al, 2014 describes the anti-inflammatory effects of WAR through interference with neutrophil adhesion and migration, a common immune response in pathogenic infection. However, as reported by Leite et al, 2016 , WAR and MeWAR were not able to decrease the production of TNF-α, IL-8 or macrophage migration inhibitory factor (MIF) in DENV-2 infected cells. This may demonstrate that besides the already known influence on cytokine secretion factors ( Spelman et al, 2006 ) by herbal immunomodulators such as Cissampelos sympodialis , direct targets on the pathogen itself can also constitute a mechanism of action of these compounds.…”

Section: Resultsmentioning

confidence: 63%

“…It cannot be ruled out that the negative antiviral results of WAR against DENV reported in the work of ( Leite et al, 2016 ) may reflect the fact that sample preparation involved the dissolution of the sample in hydrochloric acid, thus forming the alkaloid hydrochloride salt that is less permeable through cell membranesOur decision to use dimethyl sulfoxide as the solvent to dissolve our samples may explain these seemingly contradictory results, such as pointed out in a previously published work by our group ( da Silva et al, 2020 ).…”

Section: Resultsmentioning

confidence: 83%

“…These include the anti-HIV-1 effect of fangchinoline ( Wan et al, 2012 ), the effect against Herpes Simplex Virus (HSV) of obamegine ( Nawawi et al, 1999 ) and the anti-influenza effect of isotetrandrine ( Zeng et al, 2006 ). The effects of C. sympodialis leaf extract and of isolated warifteine against Dengue virus was previously studied ( Leite et al, 2016 ). More recently, our group reported the antiviral activity of alkaloids from Cissampelos sympodialis against serotype II Dengue virus.…”

Section: Introductionmentioning

confidence: 99%

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Bisbenzylisoquinoline Alkaloids of Cissampelos Sympodialis With in Vitro Antiviral Activity Against Zika Virus

et al. 2021

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In search of new antiviral compounds against Zika virus we conducted a bioassay-guided fractionation of bisbenzyilisoquinoline alkaloids isolated from Cissampelos sympodialis (Menispermaceae), a medicinal plant species endemic to Brazil. Six subfractions were obtained from a tertiary alkaloidal fraction of the rhizomes (TAFrz) using preparative high-performance liquid chromatography. All the subfractions were tested against Zika virus-infected Vero cells as the cellular model to evaluate cytotoxicity and antiviral effective concentrations. The results showed that three of the six TAFrz subfractions tested were active. The most active ones were the subfraction 6 (that consisted of the alkaloids methylwarifteine and warifteine present as a mixture at a ratio of 8.8:1.2 respectively) and the subfraction 5, that was later identified as warifteine, the major tertiary alkaloid of this species. Warifteine was able to significantly reduce virus titer in Zika virus-infected Vero cells with an IC50 of 2.2 μg/ml and this effect was selective (selectivity index, SI = 68.3). Subfraction 6 had an IC50 = 3.5 μg/ml and was more cytotoxic than pure warifteine, with SI = 6.14. Fraction 5 and fraction 6 were more potent in decreasing the viral titer of Zika virus-infected Vero cells than 6-methylmercaptopurine riboside (IC50 = 24.5 μg/ml and SI = 11.9), a mercaptopurine riboside with ZIKV antiviral activity used as a positive control. Our data demonstrate that alkaloids of the bisbenzylisoquinoline type may be explored as new antiviral agents or as an useful pharmacophore for investigating ZIKV antiviral activity.

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Section: Resultsmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

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Bisbenzylisoquinoline Alkaloids of Cissampelos Sympodialis With in Vitro Antiviral Activity Against Zika Virus

et al. 2021

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“…Further isolation and elucidation of two AFL alkaloids were found to have no activity, therefore eliminating them as the components responsible for AFL’s activity. Further research must be done to isolate the active components of this fraction to give a clear mechanism of action for AFL [ 92 ]. A list of plant species with IC 50 /EC 50 data is summarized in Table 5 .…”

Section: Antiviral Activity From Plant Extracts and Secondary Metabolitesmentioning

confidence: 99%

Plant-Based Natural Products and Extracts: Potential Source to Develop New Antiviral Drug Candidates

et al. 2021

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Viral infections are among the most complex medical problems and have been a major threat to the economy and global health. Several epidemics and pandemics have occurred due to viruses, which has led to a significant increase in mortality and morbidity rates. Natural products have always been an inspiration and source for new drug development because of their various uses. Among all-natural sources, plant sources are the most dominant for the discovery of new therapeutic agents due to their chemical and structural diversity. Despite the traditional use and potential source for drug development, natural products have gained little attention from large pharmaceutical industries. Several plant extracts and isolated compounds have been extensively studied and explored for antiviral properties against different strains of viruses. In this review, we have compiled antiviral plant extracts and natural products isolated from plants reported since 2015.

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Cissampelos genus: biological activities, ethnobotanical and phytochemical aspects

et al. 2020

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Cissampelos sympodialis has anti-viral effect inhibiting dengue non-structural viral protein-1 and pro-inflammatory mediators

Cited by 7 publications

References 20 publications

Bisbenzylisoquinoline Alkaloids of Cissampelos Sympodialis With in Vitro Antiviral Activity Against Zika Virus

Bisbenzylisoquinoline Alkaloids of Cissampelos Sympodialis With in Vitro Antiviral Activity Against Zika Virus

Plant-Based Natural Products and Extracts: Potential Source to Develop New Antiviral Drug Candidates

Cissampelos genus: biological activities, ethnobotanical and phytochemical aspects

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