Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii products

Abstract

Please cite this article in press as: Beringhs, A.O., et al. Response Surface Methodology IV-Optimal design applied to the performance improvement of an RP-HPLC-UV method for the quantification of phenolic acids in Cecropia glaziovii products. Revista Brasileira de Farmacognosia (2015), http://dx.

Association of PLGA Microspheres to Carrier Pellets by Fluid Bed Coating: A Novel Approach towards Improving the Flowability of Microparticles

“…Commercially-available microcrystalline cellulose 101 pellets (1,000 μ m average size) manufactured by extrusion-spheronization (Microcel® Pellets) were donated by Blanver (Brazil). Cecropia glaziovii leaves were purchased from the Pluridisciplinary Center of Chemical, Biological, and Agronomic Studies of the University of Campinas (Brazil) and the freeze-dried extract employed as model drug was prepared as previously described in the literature [ 20 ]. All other reagents and solvents were of analytical grade.…”

“…Chromatographic analyses were performed to quantify chlorogenic acid (CGA), a chemical marker of C. glaziovii extracts. A previously validated method [ 20 ] was employed using a PerkinElmer Series 200 chromatographer. Stationary phase was a Luna C18(2) column (5 μ m, 150 x 4.6 mm, 100 Å; Phenomenex, USA).…”

“…Cecropia glaziovii freeze-dried extract was employed as model drug due to its high hygroscopicity, which leads to an increase in capillary forces acting on the particles loading it [ 14 ]. C. glaziovii is a highly bioactive vegetal species [ 15 – 19 ] and it has been studied before as a bioactive product for pharmaceutical purposes [ 20 , 21 ]. In the coating studies, two hydrophilic polymers (Kollidon® VA64 and Kollicoat® Protect) were assessed as coating materials to attach microspheres to the surface of carrier pellets.…”

Employing an R Software Package rsm for Optimizing of Genistein, Daidzein, and Glycitein Separation and Its Application for Soy Milk Analysis by HPLC Method

“…On the other hand, R, as opensource software with the GNU General Public License (GPL), offers a broad range of statistical technique by installing software packages for each statistical need [22]. The interest in implementing RSM for the HPLC method has increased since there were many reports on its application in the field of chromatographic method optimization over the last decade [23][24][25][26][27][28]. Nevertheless, there was limited publication reporting the employment of R software for HPLC optimization.…”

Micellar electrokinetic capillary chromatography fingerprints combined with multivariate statistical analyses to evaluate the quality consistency and predict the fingerprint–efficacy relationship of Salviae miltiorrhizae Radix et Rhizoma (Danshen)

“…In fact, if interactions between factors exist, then simultaneous variations of these factors should be considered to optimize of BGE to the greatest extent. In the respect, the response surface methodology (RSM) basing upon IV‐Optimal algorithm is applied to dispose multiple factors to simultaneous optimization, which is a multivariate technique, using a set of mathematical and statistical tools, to calculate a fitting polynomial equation to the experimental data . It is also appropriate for the extrapolation model to predict the factors, as well as their possible interactions, which may influence the different experimental responses .…”