Micellar electrokinetic capillary chromatography fingerprints combined with multivariate statistical analyses to evaluate the quality consistency and predict the fingerprint–efficacy relationship of Salviae miltiorrhizae Radix et Rhizoma (Danshen)

Abstract

Micellar electrokinetic capillary chromatography fingerprinting combined with quantification was successfully established and applied to evaluate the quality consistency of Danshen, which is a medicinal herb used to treat various diseases, especially coronary cerebrovascular diseases. A background electrolyte composed of 20 mmol/L sodium tetraborate, 90 mmol/L orthoboric acid, 25 mmol/L sodium phosphate monobasic dehydrate, and 65 mmol/L sodium dodecyl sulfate was used to separate compounds. To optimize micellar electrokinetic capillary chromatography conditions, a response surface strategy was set up for orthogonal experimental design. In fingerprint assessments, a systematic quantified fingerprint method was established for integrated quality assessment of Danshen samples from qualitative and quantitative perspectives, by which the quality of 30 samples was well differentiated. The principal component analysis coupled with quantitative determination of two components was applied to explain that the quality consistency of the medicinal herb was relatively good within one harvest season, but poor among harvest seasons for the Danshen samples. In addition, the fingerprint-efficacy relationship between the chemical fingerprints and antioxidant activities was investigated utilizing orthogonal projection to latent structures, which provided important medicinal efficacy information for quality control. This work offered an efficient, holistic, and powerful approach to evaluate the quality consistency of Danshen samples.

Assessment of quality consistency in traditional Chinese medicine using multi‐wavelength fusion profiling by integrated quantitative fingerprint method: Niuhuang Jiedu pill as an example

“…First, sample and reference fingerprint vectors are respectively converted into and , the cosine of the two vectors after the conversion is denoted as the qualitative ratio similarity (), as calculated by Eq. . This conversion makes all the peaks in the same range.…”

UHPLC-ESI-Q-TOF-MS/MS analysis, antioxidant activity combined fingerprints for quality consistency evaluation of compound liquorice tablets

“…On the other hand, conventional chromatographic ngerprint analysis is typically used for authenticity and identication, but this only indicates the qualitative similarity and ignores the quantitative assessment. 20,21 Although, the quantitative evaluation of chromatographic ngerprinting in the QC of CHM has been veried; [22][23][24] nevertheless, multi-component quantication is not credible in situations where certain medicinal ingredients are ignored. In this study, both the averaged linear qualitative and averaged quantitative similarities of the ngerprints of the CLT samples were assessed by the averaged linear quantied ngerprint method (ALQFM), which was developed and successfully applied to address the issue of performing a qualitative and quantitative comparison of the reference standards and PPCE samples.…”

Identification of Confusable Herbal Medicines by Mapping of Partial Degradation Products from Herbal Medicine Polysaccharides

“…2 At present, in addition to the traditional methods for HM identification, such as macroscopic and microscopic identification, 3 thin layer chromatography (TLC) identification, 4 infrared radiation (IR) identification, 5 and ultraviolet (UV) and fluorescent light (FL) identification, 6 molecular identification using DNA barcode 7 and fingerprint profiling of small molecular ingredients in HM are now widely used comprehensive techniques. 8,9 As an important active ingredient in HMs, polysaccharide has attracted increasing attention in therapy for its natural and secure potency, such as immunomodulatory, antitumor, anti-inflammatory, anti-aging, and so on. 10 It has high genetic stability and contains the characteristic information of the species.…”