Hepatoprotective effect of Ficus religiosa latex on cisplatin induced liver injury in Wistar rats

Abstract

Revista Brasileira de Farmacognosia xxx (2015) xxx-xxx w w w . s b f g n o s i a . o r g . b r / r e v i s t aOriginal Article a b s t r a c t Ficus religiosa L., Moraceae, is widely planted in the tropics. The chemical constituents of F. religiosa include tannin, saponin gluanol acetate, ␤-sitosterol, leucoanthocyanidin, and leucoanthocyanin. These are used for the treatment of pain, inflammation, impotence, menstrual disturbances, and urine related problems, and as uterine tonic. The present study aimed to evaluate hepatoprotective effects of F. religiosa latex on cisplatin induced liver injury in Wistar rats. In experimental protocol contained five groups of rats (n = 6). In which, group I (control) was administered acacia (2%, w/v) of 5 ml/kg throughout the experiment for 16 days. The group II (cisplatin treated) was administered single dose of cisplatin (7.5 mg/kg i.p.) on 1 st day. Group III (extract control) was administered 300 mg/kg p.o. of extract for 1 st to 10 th day. Group IV (Protective) was administered extract (300 mg/kg p.o.) of F. religiosa latex for 1 st to 10 th day and administered single dose of cisplatin (7.5 mg/kg i.p.) on 11 th day and group V (Curative) received single dose of cisplatin (7.5 mg/kg i.p.) on day 1 st , and administered extract (300 mg/kg p.o.) from 7 th to 16 th days. On the 6 th day in cisplatin treated, 10 th day in extract control and 16 th day in control, protective and curative, blood withdrawn from retro-orbital sinus of rats for biochemical estimation for serum and dissected out the livers for estimation of antioxidant enzymes and histopathological works. The cisplatin-treated group 2 showed a significant increase in serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and hepatocytes cells degeneration inflammatory infiltrate and necrosis it's were significantly (**p < 0.01) alleviates by protective groups.

Influence of L-arginine on Cisplatin-Induced Hepatotoxicity in both normally Fed and Protein Malnourished Rats

“…These results are in agreement with the study reported by Naqshbandi et al [51]. This observation is consistent with a recent report by Yadav, (2015) that showed CDDP administration induced significant increase in serum ALT, AST and significant decrease in serum total protein, and albumin levels [30]. The ability of CDDP to cause alterations in the activity of these enzymes could be a secondary event following CDDP-induced liver damage with the consequent leakage from hepatocytes.…”

“…with isotonic saline solution (vehicle of CDDP), (2) CDDP group: rats were administered single dose of CDDP (7.5 mg/ kg i.p. ), [30] on the 1st day, (3) Sly group (curative): rats were administered single dose of CDDP (7.5 mg/ kg ip) on the 1st day and administered Sly at a dose (100 mg/ kg i.p. ), [31], for 5 consecutive days, (4) Larginine group (curative) : rats were administered single dose of CDDP (7.5 mg/ kg i.p.)…”

Pharmacology of Ficus religiosa- A review

“…Orally administered dose ranged from 50-2000 mg/kg of extract did not produce any significant changes in the autonomic or behavior responses in rats [56].In acute oral toxicity studies, the Ficus religiosa extract treated rats were observed for mortality up to 48 hrs. There was no mortality or any signs of behavioral changes observed after oral administration of methanol extract up to 5000 mg/kg body weight [95].…”

“…Cisplatin-treated animals showed significant increase in serum alanine amino transferase, aspartate amino transferase, alkaline phosphatase and hepatocytes cells degeneration, inflammatory infiltrate and necrosis, these changes were significantly (p < 0.01) alleviates by Ficus religiosa latex [56].Alcohol extract of Ficus religiosa stem bark was evaluated for nephro-protective effects, it reduced the blood urea nitrogen level close to normal value against the toxic effects induced by rifampicin and isoniazid in rabbits. The histopathological studies of kidney of untreated rabbit showed severe degree of infiltration in the glomerulus without renal tubular space between the glomerulus, congestion in the renal parenchyma, necrosis and condensed nucleus.…”

Natural Products for Prevention and Treatment of Chemical‐Induced Liver Injuries

“…For injuries induced by other drugs, various medicinal herbs have been found to be hepatoprotective. The hepatotoxicity caused by cisplatin was reported to be alleviated by Ficus religiosa L. extract, as shown for reversed hepatic biomarkers and reduced liver cell degeneration, inflammatory infiltration, and necrosis (Yadav, ). Liver protection was also observed in a rat model with chlorpromazine, a common antipsychotic medication, via regulating the metabolism of lipids, bile acids, and free fatty acids, when the rats were fed with the traditional Chinese medicine formula Yin‐Chen‐Hao‐Tang ( Artemisia capillaris Thunb, Gardenia jasminoides Ellis, and Rheum officinale Baill; Yang et al., ).…”