Seasonal influence and cytotoxicity of extracts, fractions and major compounds from Allamanda schottii

Abstract

Chromatography Iridoids K562 and Nalm6 cells Plumericin A B S T R A C T The aim of this research was to evaluate the fractions obtained from the leaf, stem and roots of Allamanda schottii, Pohl, Apocynaceae, responsible for the cytotoxicity, using several cell lines. Cytotoxicity was correlated with the season the part of the plant, and the major compounds were assessed. The ethanol extracts of leaves, stems and roots obtained at different seasons were evaluated in the human erythromyeloblastoid leukemia cell line (K562). Subsequently the ethanol extracts and dichloromethane fractions collected in winter were evaluated in mouse fibroblast cell line (Mus musculus) (L929), cervix adenocarcinoma (HeLa), human pre-B leukemia (Nalm6), as well as K562 cell line. The compounds plumericin, plumieride and ursolic acid isolated from ethanol extracts of the stems were evaluated in the same cell lines, as well as on breast adenocarcinoma cell line (MCF-7), and Mus musculus skin melanoma cell line (B16F10). The chromatographic profiles of the dichloromethane fractions were obtained by high performance liquid chromatography. The results revealed that the season during which A. schottii was collected, and the part of the plant analyzed, influence the cytotoxicity on the K562 cells tested. On the other hand the dichloromethane fractions, mainly from the stems and roots, are responsible for the cytoxicity on the cells tested. These results may be associated with the seasonal variation of plumericin in these parts of the plant. This information is in accordance with the HPLC analysis. The resultsclearly show the potential for the phytotherapeutic use of this species, and suggest that the cytotoxic activity observed may be due to the presence of plumericin, or to minor com-pounds not yet identified. The seasonal influence on the production of secondary metabolites was verified.

Antioxidant and cicatrizing activity of the species Abarema cochliacarpos (Gomes) Barneby J. W. Grimes

“…Results of in vitro tests were expressed in EC50 values (Nascimento et al, 2014), which is the concentration required to produce half (50%) of maximum effect estimated at 100% for the plant extract.…”

Avaliação de efeitos preliminares do plumierídeo após administração oral a camundongos

“…A sua estrutura química é composta por um anel ciclopentano-pirano e um glicosídeo (conforme figura 1), o que confere alta solubilidade na molécula (SIMÕES et al, 2003). Seu isolamento já foi registrado a partir dos gêneros Plumeria e Plantago, além da Allamanda cathartica (CHATTERJEE et al, 2012;NASCIMENTO et al, 2014;PANKOKE et al, 2013). Silva (2007) relata pronunciados efeitos do iridoide (0,0001-1 µg/Kg) sob quadros de inflamação e dor induzidos por carragenina em camundongos e ratos, após administração oral.…”

DEVELOPMENT AND OPTIMIZATION OF A MICROWAVE-ASSISTED EXTRACTION OF PLUMIERIDE FROM Allamanda cathartica L. FLOWERS

“…The purity of the compound was assessed by high performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) analysis, through comparison with the literature and direct comparison with original samples. 13,21,35 Plumieride presented a purity > 98%. Methanol and acetonitrile were purchased from Tedia® (HPLC grade).…”

“…The compound was analyzed by HPLC and NMR, then the data were compared with those found in the literature or with data of the standard previously isolated. 13,21,35 …”

“…13,[15][16][17][18][19] The iridoid plumieride has been isolate from several genus like Allamanda, Himatanthus, Plantago and Plumeria in different yields. [20][21][22][23][24][25] Plumieride shows antidermatophytic, antifungal, plant growth inhibitory potential and in vitro cytotoxicity against radiationinduced fibrosarcoma (RIF) tumor cells. 19,20,26 Extraction of secondary metabolites with pharmacological activity has been investigated in recent decades, focusing mainly on extraction with a conventional solvent.…”