The effect of melatonin and vitamin C treatment on the experimentally induced tympanosclerosis: study in rats

Koç, Sema; Kiyici, Halil; Toker, Aysun; Soyalıç, Harun; Aslan, Hüseyin; Kesici, Hakan; Karaca, Zafer İsmail

doi:10.1016/j.bjorl.2016.06.008

Cited by 2 publications

(2 citation statements)

References 25 publications

(36 reference statements)

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“…Three days after injection of inactivated S. pneumoniae, yellowish serous fluid was found in the middle ear cavity of most rats, but there was generally no purulent fluid. Long-term exudation of inflammatory substances stimulates tympanosclerosis in the middle ear mucosa; meanwhile, perforation of the tympanic membrane during injection promotes the formation of a high-oxygen environment in the tympanum, which in turn induces accumulation of oxygen free radicals and promotes tympanosclerosis (30). Perforation of the tympanic membrane caused by puncture usually heals within 2 weeks.…”

Section: Discussionmentioning

confidence: 99%

Role of TGF‑β1‑mediated epithelial‑mesenchymal transition in the pathogenesis of tympanosclerosis

et al. 2020

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The present study aimed to explore the role of TGF-β1-mediated epithelial-mesenchymal transition (EMT) in the pathogenesis of tympanosclerosis. Sprague Dawley rats were injected with inactivated Streptococcus pneumoniae suspension to establish a rat model of tympanosclerosis. The rats were sacrificed 8 weeks after the model was established. H&E and von Kossa staining was used to observe the morphological changes of middle ear mucosa. Western blotting was used to detect the expression of TGF-β1 and EMT-associated proteins in the mucosa samples. Middle ear mucosal epithelial cells of rats were collected to establish a primary culture. The cultured cells were stimulated with TGF-β1 and the expression of EMT-associated proteins was detected by western blotting and immunofluorescence. In addition, the cells were treated with TGF-β receptor type I/II inhibitor and the expression level of EMT-associated proteins was detected by western blotting. Sclerotic lesions appeared on 72.4% of tympanic membranes, and marked inflammation, inflammatory cell infiltration and fibrosis were found in the middle ear mucosa of rat models of tympanosclerosis. In middle ear mucosa of rats with tympanosclerosis, the expression of mesenchymal cell markers increased and that of epithelial cell markers decreased compared with the control group. TGF-β1 stimulated the activation of the EMT pathway in middle ear mucosal epithelial cells, resulting in an increased expression of fibronectin and N-cadherin. In addition, a decreased expression level of EMT-associated proteins was observed when TGF-β1 was inhibited. In conclusion, the present study indicated that TGF-β1-mediated EMT may play an important role in the pathogenesis of tympanosclerosis.

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Section: Discussionmentioning

confidence: 99%

Role of TGF‑β1‑mediated epithelial‑mesenchymal transition in the pathogenesis of tympanosclerosis

et al. 2020

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“…Although we have not measured oxidative stress markers in the present study, it has been shown that formation of free oxygen radicals is important for the development of tympanosclerosis within the middle ear (3). Researches have emphasized that melatonin, a potent antioxidant, inhibits sclerosis (11). Hence, antioxidant treatment may reduce tympanosclerosis formation.…”

Section: Discussionmentioning

confidence: 99%

Systemic inflammatory blood markers in patients with tympanosclerosis

Düzenli¹,

Bozan²,

Akin³

et al. 2019

Eastern J Med

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Tympanosclerosis is a disorder as known hyaline accumulation in the middle ear. Inflammation has been proposed in the development of tympanosclerosis. The mean platelet volume (MPV), neutrophil-to-lymphocyte ratio (NLR) and red cell distribution width (RDW) are most common markers of systemic inflammation. Fourty patients who diagnosed tympanosclerosis intraoperatively and 35 subjects without tympanosclerosis were enrolled in this retrospective study. The MPV, RDW, neutrophil and lymphocyte as well as platelet counts were evaluated from complete blood counts in both trial participants. NLR was calculated.

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The effect of melatonin and vitamin C treatment on the experimentally induced tympanosclerosis: study in rats

Abstract: Melatonin increases total antioxidant status level and might have some effect on tympanosclerosis that develops after myringotomy.

Cited by 2 publications

References 25 publications

Role of TGF‑β1‑mediated epithelial‑mesenchymal transition in the pathogenesis of tympanosclerosis

Role of TGF‑β1‑mediated epithelial‑mesenchymal transition in the pathogenesis of tympanosclerosis

Systemic inflammatory blood markers in patients with tympanosclerosis

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The effect of melatonin and vitamin C treatment on the experimentally induced tympanosclerosis: study in rats

Abstract: Melatonin increases total antioxidant status level and might have some effect on tympanosclerosis that develops after myringotomy.

Cited by 2 publications

References 25 publications

Role of TGF‑&beta;1‑mediated epithelial‑mesenchymal transition in the pathogenesis of tympanosclerosis

Role of TGF‑&beta;1‑mediated epithelial‑mesenchymal transition in the pathogenesis of tympanosclerosis

Systemic inflammatory blood markers in patients with tympanosclerosis

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Role of TGF‑β1‑mediated epithelial‑mesenchymal transition in the pathogenesis of tympanosclerosis

Role of TGF‑β1‑mediated epithelial‑mesenchymal transition in the pathogenesis of tympanosclerosis