Role of TGF‑&amp;beta;1‑mediated epithelial‑mesenchymal transition in the pathogenesis of tympanosclerosis

Qiu, Jingjun; Wang, Yanmei; Guo, Wentao; Xu, Ling; Mou, Yakui; Cui, Limei; Han, Fengchan; Sun, Yan

doi:10.3892/etm.2020.9438

Cited by 8 publications

(6 citation statements)

References 41 publications

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“…Oil-infused silicone also greatly limited the deposition of fibronectin (fig. S15), which is associated with the TM inflammatory response that can lead to biofilm formation, myringitis, and rejection of the device ( 46 , 47 ).…”

Section: Resultsmentioning

confidence: 99%

Design of medical tympanostomy conduits with selective fluid transport properties

et al. 2023

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Implantable tubes, shunts, and other medical conduits are crucial for treating a wide range of conditions from ears and eyes to brain and liver but often impose serious risks of device infection, obstruction, migration, unreliable function, and tissue damage. Efforts to alleviate these complications remain at an impasse because of fundamentally conflicting design requirements: Millimeter-scale size is required to minimize invasiveness but exacerbates occlusion and malfunction. Here, we present a rational design strategy that reconciles these trade-offs in an implantable tube that is even smaller than the current standard of care. Using tympanostomy tubes (ear tubes) as an exemplary case, we developed an iterative screening algorithm and show how unique curved lumen geometries of the liquid-infused conduit can be designed to co-optimize drug delivery, effusion drainage, water resistance, and biocontamination/ingrowth prevention in a single subcapillary–length-scale device. Through extensive in vitro studies, we demonstrate that the engineered tubes enabled selective uni- and bidirectional fluid transport; nearly eliminated adhesion and growth of common pathogenic bacteria, blood, and cells; and prevented tissue ingrowth. The engineered tubes also enabled complete eardrum healing and hearing preservation and exhibited more efficient and rapid antibiotic delivery to the middle ear in healthy chinchillas compared with current tympanostomy tubes, without resulting in ototoxicity at up to 24 weeks. The design principle and optimization algorithm presented here may enable tubes to be customized for a wide range of patient needs.

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Section: Resultsmentioning

confidence: 99%

Design of medical tympanostomy conduits with selective fluid transport properties

et al. 2023

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“…These observations suggest S. lutetiensis promoted ccRCC development, but the underlying mechanisms were unclear. Previous studies reported that some Streptococcus species activated TGF -β signaling in various diseases, e.g., Streptococcus pneumoniae induced TGF -β1 expression during tympanosclerosis pathogenesis ( Qiu et al, 2021 ), asthma inflammation ( Schmit et al, 2020 ) and pulmonary infection ( Peteranderl et al, 2017 ). So we theorized that S. lutetiensis promoted ccRCC via TGF -β signaling, to explore this possibility, the ACHN cell line was co-cultured with Sl.CM or BHI and the mRNA expression levels of the TGF -β signaling pathway were assessed by qRT-PCR.…”

Section: Resultsmentioning

confidence: 99%

Characteristics of Gut Microbiota in Patients With Clear Cell Renal Cell Carcinoma

Yang

Dong

et al. 2022

Front. Microbiol.

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Different gut microbiota is implicated in different diseases, including cancer. However, gut microbiota differences between individuals with clear cell renal cell carcinoma (ccRCC) and healthy individuals are unclear. Here, we analyzed gut microbiota composition in 51 ccRCC patients and 40 healthy controls using 16S rRNA sequencing analysis. We observed that Blautia, Streptococcus, [Ruminococcus]_torques_group, Romboutsia, and [Eubacterium]_hallii_group were dominant and positively associated with ccRCC. We isolated and cultured Streptococcus lutetiensis to characterize specific gut microbiota that promotes ccRCC and found that it promoted in vitro ccRCC proliferation, migration, and invasion via the TGF-signaling pathway. Interactions identified between the gut microbiota and ccRCC suggest the gut microbiota could serve as a potential non-invasive tool for predicting ccRCC risk and also function as a cancer therapy target.

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“…Pathologic EMT can be triggered by lymphocyte activation and secretion of transforming growth factor-beta (TGF-b). Mouse models with knockout of TGF-b show protection from fibrosis and EMT (153)(154)(155). Growth factors such as VEGF and TGF-b also mediate interactions between the lung endothelium and pericytes and have been independently studied as drivers of fibrosis (156,157).…”

Section: Airway Inflammation In the Pathogenesis Of Cladmentioning

confidence: 99%

Lymphocytic Airway Inflammation in Lung Allografts

2022

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Lung transplant remains a key therapeutic option for patients with end stage lung disease but short- and long-term survival lag other solid organ transplants. Early ischemia-reperfusion injury in the form of primary graft dysfunction (PGD) and acute cellular rejection are risk factors for chronic lung allograft dysfunction (CLAD), a syndrome of airway and parenchymal fibrosis that is the major barrier to long term survival. An increasing body of research suggests lymphocytic airway inflammation plays a significant role in these important clinical syndromes. Cytotoxic T cells are observed in airway rejection, and transcriptional analysis of airways reveal common cytotoxic gene patterns across solid organ transplant rejection. Natural killer (NK) cells have also been implicated in the early allograft damage response to PGD, acute rejection, cytomegalovirus, and CLAD. This review will examine the roles of lymphocytic airway inflammation across the lifespan of the allograft, including: 1) The contribution of innate lymphocytes to PGD and the impact of PGD on the adaptive immune response. 2) Acute cellular rejection pathologies and the limitations in identifying airway inflammation by transbronchial biopsy. 3) Potentiators of airway inflammation and heterologous immunity, such as respiratory infections, aspiration, and the airway microbiome. 4) Airway contributions to CLAD pathogenesis, including epithelial to mesenchymal transition (EMT), club cell loss, and the evolution from constrictive bronchiolitis to parenchymal fibrosis. 5) Protective mechanisms of fibrosis involving regulatory T cells. In summary, this review will examine our current understanding of the complex interplay between the transplanted airway epithelium, lymphocytic airway infiltration, and rejection pathologies.

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Role of TGF‑β1‑mediated epithelial‑mesenchymal transition in the pathogenesis of tympanosclerosis

Cited by 8 publications

References 41 publications

Design of medical tympanostomy conduits with selective fluid transport properties

Design of medical tympanostomy conduits with selective fluid transport properties

Characteristics of Gut Microbiota in Patients With Clear Cell Renal Cell Carcinoma

Lymphocytic Airway Inflammation in Lung Allografts

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