Tricalcium phosphate solubilization and nitrogen fixation by newly isolated Aneurinibacillus aneurinilyticus CKMV1 from rhizosphere of Valeriana jatamansi and its growth promotional effect

Chauhan, Anjali; Guleria, Shiwani; Balgir, Praveen P.; Walia, Abhishek; Mahajan, Rishi; Mehta, Preeti; Shirkot, C. K.

doi:10.1016/j.bjm.2016.12.001

Cited by 64 publications

(19 citation statements)

References 33 publications

(49 reference statements)

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“…These isolates may have an additional benefit of acclimatization to the acidic condition of the soil in tea cultivation and may augment plant microbe interactions. Sharma et al (2017) and Chauhan et al () previously reported the importance of beneficial micro‐organisms in soil fertility and plant growth.…”

Section: Discussionmentioning

confidence: 99%

Culturable endophytic bacteria ofCamelliaspecies endowed with plant growth promoting characteristics

et al. 2019

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Aim: Tea (Camellia sinensis (L.) O. Kuntze) is an economically important caffeine-containing beverage crop with massive plantation in the Northeast corner of the agroclimatic belt of India. The main aim of the work was to isolate, identify and characterize the native plant growth promoting endophytes associated with tea for future microbe based bioformulation. Methods and Results: A total of 129 endophytic bacteria were isolated and characterized for plant growth promoting traits such as indole-3-acetic acid (IAA), phosphate solubilization, ammonia production, biocontrol traits like siderophore and extracellular enzyme production. BOX-PCR fingerprinting was used to differentiate the various bacterial isolates obtained from six different tea species. 16S rRNA sequencing and BLAST analysis showed that these isolates belonged to different genera, that is, Bacillus, Brevibacterium, Paenibacillus and Lysinibacillus. Lysinibacillus sp. S24 showed the highest phosphate solubilization and IAA acid production efficiency of 268Á4 AE 14Á3 and 13Á5 AE 0Á5 µg ml À1 , respectively. Brevibacterium sp. S91 showed the highest ammonia production of 6Á2 AE 0Á5 µmol ml À1 . Chitinase, cellulase, protease and pectinase activities were shown by 4Á6, 34Á1, 27Á13 and 13Á14% of the total isolates, respectively. Similarly, 41% of the total isolates were positive for 1-aminocyclopropane-1carboxylic acid (ACC) deaminase activity. Further, the potent PGP isolates, S24 and S91 were able to enhance the vegetative parameters such as dry/fresh weight of root and shoot of tea plants in nursery conditions. Conclusion: Our findings corroborate that tea endophytic bacteria possess the potential to demonstrate multiple PGP traits both, in vivo and in vitro and have the potential for further large-scale trials. Significance and Impact of the Study: The exploration of tea endophytic bacterial community is suitable for the development of bioformulations for an integrated nutrient management and thus sustainable crop production and decreasing the hazardous effects of chemical fertilizers on the environment and human health.

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Section: Discussionmentioning

confidence: 99%

Culturable endophytic bacteria ofCamelliaspecies endowed with plant growth promoting characteristics

et al. 2019

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“…strain SG_AIOA2, respectively. There are few reports available in the literature documenting the ACC deaminase producing ability and PGPR potential of Aneurinibacillus and Paenibacillus (Goswami et al, 2015; Chauhan et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

ACC Deaminase Producing Bacteria With Multifarious Plant Growth Promoting Traits Alleviates Salinity Stress in French Bean (Phaseolus vulgaris) Plants

2019

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Plant growth promoting rhizobacteria (PGPR) with 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity has the potential to promote plant growth and development under adverse environmental conditions. In the present study, rhizobacterial strains were isolated from Garlic ( Allium sativum ) rhizosphere and were screened in vitro ACC deaminase activity in DF salt minimal media supplemented with 3 mM ACC. Out of six isolates, two could degrade ACC into α-ketobutyrate, exhibiting ACC deaminase activity producing more than ∼1500 nmol of α-ketobutyrate mg protein -1 h -1 , and assessed for other plant growth promoting (PGP) functions including indole acetic acid production (greater than ∼30 μg/ml), siderophore, Ammonia, Hydrogen cyanide production and inorganic Ca 3 (PO 4 ) 2 (∼85 mg/L) and ZnSO 4 solubilization. Besides facilitating multifarious PGP activities, these two isolates augmented in vitro stress tolerance in response to 6% w/v NaCl salt stress and drought stress (-0.73 Mpa). The strains ACC02 and ACC06 were identified Aneurinibacillus aneurinilyticus and Paenibacillus sp., respectively on the basis of 16S rDNA gene sequence analysis and were evaluated for growth promoting potential in French bean seedlings under non-saline and salinity stress conditions through pot experiments. The seed bacterization by ACC02 and ACC06 revealed that treatment of plants with bacterial isolates in the form of consortia significantly declined (∼60%) stress stimulated ethylene levels and its associated growth inhibition by virtue of their ACC deaminase activity. The consortia treatment alleviated the negative effects of salinity stress and increased root length (110%), root fresh weight (∼45%), shoot length (60%), shoot fresh weight (255%), root biomass (220%), shoot biomass (425%), and total chlorophyll content (∼57%) of French bean seedlings subjected to salinity stress.

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“…A partir del descubrimiento de Schoöllhorn, Burris, y Dilworth en 1966(Dilworth, 1966Schoöllhorn & Burris, 1967) acerca de que la nitrogenasa reduce el acetileno (C 2 H 2 ) a etileno (C 2 H 4 ), se sugirió que la tasa de reducción de acetileno se puede usar como índice de la tasa de fijación de nitrógeno (Stewart, Fitzgerald, Burris, & Nov, 1967). Desde entonces, se utiliza esta técnica en la que se requiere de un cromatógrafo de gases y se reemplaza 10-20 % (Chauhan et al, 2017) del volumen del aire por acetileno en una cámara de incubación que se deja a 28-30 °C durante 2 a 5 días (Chauhan et al, 2017;Das & De, 2018;Yong et al, 2018). Asimismo, se pueden analizar plantas completas, suelo, muestras de nódulos y de bacterias (Camelo-Rusinque, Moreno-Galván, Romero-Perdomo, & Bonilla-Buitrago, 2017).…”

Section: Técnica De Abundancia Natural (δ 15 N)unclassified

“…Cuando la muestra corresponde a bacterias, las cepas se cultivan en medios semisólidos (Nfb) en viales (García et al, 2017) por 5 días a 28 °C y se exponen a atmósferas con 10 % de acetileno durante 2 a 5 días; posteriormente, se hace la extracción de 1 mL de muestra y se analiza por cromatografía de gases (Chauhan et al, 2017). Una de las mayores limitantes en esta técnica se relaciona con la integridad de los nódulos de la planta para que no se altere la actividad de la nitrogenasa (Chalk & Craswell, 2018).…”

Section: Técnica De Abundancia Natural (δ 15 N)unclassified

Técnicas aplicadas en la investigación agrícola para cuantificar la fijación de nitrógeno: una revisión sistemática

Fonseca-López

Quila

Balaguera-López

2019

CTA

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La economía del nitrógeno es un tema que viene creciendo a nivel mundial, especialmente en la actividad agrícola cuando por mal manejo de la fertilización ocurren pérdidas por lixiviación que contribuyen a la eutrofización del agua, lo que incrementa la población de algas y, como efecto, reduce la disponibilidad de oxígeno, acelerando el proceso de desnitrificación en el que se produce N2O, conocido por su efecto en el calentamiento global. Igualmente, parte del fertilizante es emitido a la atmósfera por volatización. Esto ha impulsado la creación de técnicas que permiten cuantificar el nitrógeno utilizado por las plantas y el fijado en el suelo a través de microrganismos para hacer más eficiente el empleo del nitrógeno en los sistemas agrícolas. Sin embargo, dentro de las mayores limitantes se encuentra su sensibilidad, especificidad, costo y tecnología que se requiere para aplicarlas. Esto ha conducido a la innovación de procedimientos y a la creación de técnicas que tienen una tasa de error muy bajo. El objetivo de este trabajo fue realizar la descripción de las principales técnicas utilizadas para cuantificar la fijación del nitrógeno con énfasis en los antecedentes, procedimientos, expresiones matemáticas que se usan, y escenarios futuros. La información se describe a partir del análisis de ensayos disponibles en la base de datos Scopus. Este trabajo consolida las técnicas que continúan vigentes para cuantificar el nitrógeno y facilita entender su uso a través del tiempo con modelos de predicción, así como su importancia, ventajas y desventajas.

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Tricalcium phosphate solubilization and nitrogen fixation by newly isolated Aneurinibacillus aneurinilyticus CKMV1 from rhizosphere of Valeriana jatamansi and its growth promotional effect

Cited by 64 publications

References 33 publications

Culturable endophytic bacteria ofCamelliaspecies endowed with plant growth promoting characteristics

Culturable endophytic bacteria ofCamelliaspecies endowed with plant growth promoting characteristics

ACC Deaminase Producing Bacteria With Multifarious Plant Growth Promoting Traits Alleviates Salinity Stress in French Bean (Phaseolus vulgaris) Plants

Técnicas aplicadas en la investigación agrícola para cuantificar la fijación de nitrógeno: una revisión sistemática

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