Genetic analysis of the first mcr-1 positive Escherichia coli isolate collected from an outpatient in Chile

Gutiérrez, Camila Fernández; Zenis, Javier; Legarraga, Paulette; Cabrera‐Pardo, Jaime R.; García, Patricia; Bello-Toledo, Helia; Opazo-Capurro, Andrés; González-Rocha, Gerardo

doi:10.1016/j.bjid.2019.05.008

Cited by 13 publications

(6 citation statements)

References 22 publications

(28 reference statements)

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“…HGT of mcr -1 is a threat to human health, so it is necessary to calculate the efficiency of mcr -1 transfer. The reported transfer efficiency of mcr -1 varies greatly depending on the experimental conditions, methods, and strains, ranging from 10 –9 to 10 –1 ( Anjum et al, 2016 ; Liu et al, 2016 ; Quesada et al, 2016 ; Gutiérrez et al, 2019 ; Lu et al, 2019 ; Li et al, 2022 ). In our study, mcr -1 from all five colistin-resistant strains was successfully transferred into the E. coli J53 recipient strain by conjugation.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Multi-Resistance Strains Carrying mcr-1 Gene Using Recombinase-Aided Amplification Directly on Clinical Samples

Fan

Feng

et al. 2022

Front. Microbiol.

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With the increasingly severe problem of bacterial resistance, colistin, as the last line of defense, has attracted attention again. Mobile colistin resistance (mcr-1) gene is involved in the horizontal transmission of colistin resistance in Gram-negative bacteria (GNB), which is a serious threat to human health. Therefore, rapid detection of mcr-1 gene presence in clinical samples is crucial. In this study, a Recombinase-aided amplification(RAA) method for mcr-1 was successfully constructed, with sensitivity of 20 copies/reaction. In addition, amplification signal could only be detected in the strain containing mcr-1 gene among 14 different bacterial species. The method was then used to test a total of 672 clinical samples from a pediatric hospital in Beijing. Five strains harbored mcr-1 genes were isolated from mcr-1-positive clinical samples and identified as Escherichia coli. Multi-locus sequence typing (MLST) analysis showed that the five E. coli belonged to different ST types. Notably, the mcr-1 gene from the isolates could be transferred conjugately to the recipient strain E. coli J53, with highest transfer efficiency up to 57–58%, suggesting that the mcr-1 gene was located on the plasmid. These findings showed that the RAA assay has potential to be a rapid and sensitive mcr-1 gene screening test for clinical samples, and mcr-1 could be transmitted vertically and horizontally between and within bacterial species in a plasmid-mediated manner.

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Section: Discussionmentioning

confidence: 99%

Rapid Detection of Multi-Resistance Strains Carrying mcr-1 Gene Using Recombinase-Aided Amplification Directly on Clinical Samples

Fan

Feng

et al. 2022

Front. Microbiol.

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“…This could be attributed to the fact that until the first report of mcr-1 gene, colistin resistance in Enterobacteriaceae was believed to be chromosomally mediated 36 . However, since 2016, the mcr-1 gene was reported in Enterobacteriaceae recovered from food, animals, and human specimens in China, and it has subsequently been reported worldwide 11, [48][49][50][51][52][53] . The wide spread of the mcr-1 dramatically challenges the newly renewed interest in colistin for clinical use and opens a new research topic on colistin.…”

Section: Discussionmentioning

confidence: 99%

Global trends and current status in colistin resistance research: a bibliometric analysis (1973-2019)

Yacouba

Olowo-Okere

2020

F1000Res

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Background: Colistin resistance is a major breach in our last line of defense and without urgent action, we are heading for a post-antibiotic era, in which common infections and minor injuries can once again kill. To the best of our knowledge, the use of the bibliometric analytical technique for examining colistin resistance-related research does not exist in the literature. Methods: Here, we analyze and present bibliometric indicators of the global literature in colistin resistance research. The Scopus database was searched for articles on colistin resistance. The articles retrieved were analyzed using the bibliometrix R-package. Results: A total of 1105 publications were retrieved. There was a noticeable increase in the number of publications on colistin resistance research in the past decade. Six journals made up the core zone in colistin research and produced 35.83% of the published articles. The analysis across time-intervals revealed several keywords that had increased or decreased in usage when comparing the interval between 1973-2009 and 2010-2019. Authors’ keywords “Acinetobacter baumanii”, and “Pseudomonas aeruginosa” were the most frequent encountered during the period of 1973-2009, while “mcr-1”, “Enterobacteriaceae”, “Escherichia coli”, and “Klebsiella pneumoniae” emerged in the past decade. Conclusions: There has been a significant growth in publications on colistin resistance in the past decade, suggesting an urgent need for action by different stakeholders to contain this threat of colistin resistance. Keyword analysis revealed temporal changes in the types of keywords used across time-intervals. These findings summarize a general vision on colistin resistance research and will serve as baseline data for future comparative purposes.

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“…Since 2012, the mcr-1 gene has already been identified in bacteria from humans, animals, animal food products, and environmental sources in different countries in Latin America, including Brazil (15), Bolivia (16), Colombia (17), Chile (18), Uruguay (19), Paraguay (20), Peru (21), Mexico (22), Venezuela (23), and Ecuador (24). Brazil is the country with the highest number of mcr-1-positive bacteria reported in Latin America mainly from bacterial isolates obtained from poultry rectal swabs (15) (Table 1).…”

Section: Mcr-1 In Latin Americamentioning

confidence: 99%

mcr-1 Gene in Latin America: How Is It Disseminated Among Humans, Animals, and the Environment?

Lentz

Dalmolin

Barth

et al. 2021

Front. Public Health

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Genetic analysis of the first mcr-1 positive Escherichia coli isolate collected from an outpatient in Chile

Cited by 13 publications

References 22 publications

Rapid Detection of Multi-Resistance Strains Carrying mcr-1 Gene Using Recombinase-Aided Amplification Directly on Clinical Samples

Rapid Detection of Multi-Resistance Strains Carrying mcr-1 Gene Using Recombinase-Aided Amplification Directly on Clinical Samples

Global trends and current status in colistin resistance research: a bibliometric analysis (1973-2019)

mcr-1 Gene in Latin America: How Is It Disseminated Among Humans, Animals, and the Environment?

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