Human sporotrichosis: recommendations from the Brazilian Society of Dermatology for the clinical, diagnostic and therapeutic management

Orofino-Costa, Rosane; Freitas, Dayvison Francis Saraiva; Bernardes-Engemann, Andréa Reis; Rodrigues, Anderson Messias; Talhari, Carolina; Ferraz, Cláudia Elise; Veasey, John Verrinder; Quintella, Leonardo Pereira; Sousa, Maria Silvia Laborne Alves de; Vettorato, Rodrigo; Almeida‐Paes, Rodrigo; Macedo, Priscila Marques de

doi:10.1016/j.abd.2022.07.001

Cited by 33 publications

(29 citation statements)

References 116 publications

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“…Sporotrichosis is a neglected fungal disease caused by thermo-dimorphic fungi belonging to the Sporothrix complex, which includes S. brasiliensis, S. schenckii sensu stricto, S. luriei and S. globosa. While those species are frequently associated with human and animal disease, the environmental species S. mexicana, S. pallida, and S. chilensis are referred to as opportunistic pathogens that cause infections in immunocompromised patients (Orofino-Costa et al, 2017;Rodrigues et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Sporotrichosis is classically transmitted by traumatic inoculation of fungal propagules into subcutaneous tissue (Orofino-Costa et al, 2022). Nevertheless, ongoing outbreaks in Brazil are mainly related to the epizootic transmission among cats infected with S. brasiliensis, a species of high virulence.…”

Section: Introductionmentioning

confidence: 99%

“…While those species are frequently associated with human and animal disease, the environmental species S. mexicana, S. pallida , and S. chilensis are referred to as opportunistic pathogens that cause infections in immunocompromised patients (Orofino-Costa et al, 2017; Rodrigues et al, 2022). Sporotrichosis is classically transmitted by traumatic inoculation of fungal propagules into subcutaneous tissue (Orofino-Costa et al, 2022). Nevertheless, ongoing outbreaks in Brazil are mainly related to the epizootic transmission among cats infected with S. brasiliensis , a species of high virulence.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, ongoing outbreaks in Brazil are mainly related to the epizootic transmission among cats infected with S. brasiliensis , a species of high virulence. Due to their close proximity to humans, zoonotic transmission from cats via scratches or bites has become a challenge for confining the geographical advance of the disease (Garcia Carnero et al, 2018; Orofino-Costa et al ., 2022; Orofino-Costa et al ., 2017). Over about 25 years, a sporotrichosis outbreak that started in the state of Rio de Janeiro has spread throughout almost all the Brazilian territory and into few of its neighbouring countries in Latin America, such as Argentina (Etchecopaz et al, 2021; Orofino-Costa et al ., 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Due to their close proximity to humans, zoonotic transmission from cats via scratches or bites has become a challenge for confining the geographical advance of the disease (Garcia Carnero et al, 2018; Orofino-Costa et al ., 2022; Orofino-Costa et al ., 2017). Over about 25 years, a sporotrichosis outbreak that started in the state of Rio de Janeiro has spread throughout almost all the Brazilian territory and into few of its neighbouring countries in Latin America, such as Argentina (Etchecopaz et al, 2021; Orofino-Costa et al ., 2022). Furthermore, at least one case of sporotrichosis, transmitted by an imported infected cat, has been reported in the United Kingdom (Rachman et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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CRISPR-based tools for genetic manipulation in pathogenicSporothrixspecies

Hatinguais

Leaves

Brown

et al. 2022

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Sporothrix brasiliensis is an emerging fungal pathogen frequently associated with zoonotic transmission of sporotrichosis. Although certain virulence factors have been proposed as potential sporotrichosis determinants, the scarcity of molecular tools for reverse genetics studies on Sporothrix has significantly impeded the dissection of mechanisms underlying the disease. Here, we demonstrate that PEG-mediated protoplast transformation is a powerful method for heterologous expression in S. brasiliensis, S. schenckii and S. chilensis. Combined with CRISPR/Cas9 gene editing, this transformation protocol allowed the deletion of the putative DHN-melanin synthase gene pks1, which is a proposed virulence factor of Sporothrix species. To improve in locus integration of deletion constructs, we deleted the KU80 homologue that is critical for non-homologous end-joining DNA repair. The use of S. brasiliensis delta ku80 strains enhanced homologous-directed repair during transformation resulting in increased targeted gene deletion. In conclusion, our CRISPR/Cas9-based transformation protocol provides an efficient tool for targeted gene manipulation in Sporothrix species.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%