Different Cell Viability Assays Reveal Inconsistent Results After Bleomycin Electrotransfer In Vitro

Jakštys, Baltramiejus; Ruzgys, Paulius; Tamošiūnas, Mindaugas; Šatkauskas, Saulius

doi:10.1007/s00232-015-9813-x

Cited by 42 publications

(18 citation statements)

References 47 publications

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“…Cell metabolic activity can change after cell treatment. MTT assay measured the cell survival rate based on the conversion of yellow MTT substrate to the purple formazan present in mitochondrial succinate dehydrogenase of metabolically active cells (Elisia, Popovich, Hu, & Kitts, ; Jakstys, Ruzgys, Tamosiunas, & Satkauskas, ). The cell survival rates with variations in the alcohol concentration are shown in Figure .…”

Section: Resultsmentioning

confidence: 99%

Discovery and identification of potential biomarkers for alcohol‐induced oxidative stress based on cellular metabolomics

Wei

Fei

et al. 2017

Biomedical Chromatography

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Biomarkers involved in alcohol-induced oxidative stress play an important role in alcoholic liver disease prevention and diagnosis. Alcohol-induced oxidative stress in human liver L-02 cells was used to discover the potential biomarkers. Metabolites from L-02 cells induced by alcohol were measured by high-performance liquid chromatography and mass spectrometry. Fourteen metabolites that allowed discrimination between control and model groups were discovered by multivariate statistical data analysis (i.e. principal components analysis, orthogonal partial least-squares discriminate analysis). Based on the retention time, UV spectrum and LC-MS findings of the samples and compared with the authentic standards, eight biomarkers involved in alcohol-induced oxidative stress, namely, malic acid, oxidized glutathione, γ-glutamyl-cysteinyl-glycine, adenosine triphosphate, phenylalanine, adenosine monophosphate, nitrotyrosine and tryptophan, were identified. These biomarkers offered important targets for disease diagnosis and other researches.

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Section: Resultsmentioning

confidence: 99%

Discovery and identification of potential biomarkers for alcohol‐induced oxidative stress based on cellular metabolomics

Wei

Fei

et al. 2017

Biomedical Chromatography

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“…Common in vitro approaches for evaluating cell response to radiation therapy include end point analyses such as the 3-[4,5-dimethylthiazolyl-2]-2,5-diphenyltetrazolium bromide (MTT) assay or the clonogenic assay. Studies evaluating cell viability after treatment can vary largely depending on the chosen end time point [19]. Additionally, these methods lack the ability to longitudinally observe cell response which is critical in determining underlying changes in cell physiology during therapy [20].…”

Section: Introductionmentioning

confidence: 99%

Quantifying the effects of combination trastuzumab and radiation therapy in human epidermal growth factor receptor 2 positive breast cancer

Bloom

Song

Virostko

et al. 2020

Preprint

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Background: Trastuzumab, a clinical antibody targeted to the human epidermal growth factor receptor 2 (HER2), has been shown to sensitize cells to radiation in vitro. Current studies lack longitudinal evaluation of cellular response and in vivo data is limited. The purpose of this study is to quantify the effects of combination trastuzumab and radiation therapy in vitro and in vivo over time to determine if there is a synergistic interaction. Methods: EGFP expressing BT474, SKBR3 and MDA-MB-231 cell lines were treated with 0.1 ng/ml of trastuzumab, 5 or 10 Gy of radiation, or combination treatment, and imaged using fluorescence live cell microscopy for one week. The Bliss independence model was used to quantify the effects of combination treatment. HER2+ tumor bearing mice (female NU/J) (N=34) were treated with saline, 10 mg/kg of trastuzumab, 5 or 10 Gy of radiation, or combination treatment. Tumor size was measured three times per week for four weeks via caliper measurements. Additional mice (N=13) were treated with 10 mg/kg of trastuzumab, 5 Gy of radiation, or combination treatment. Tumors were harvested at one week and evaluated with immunohistochemistry for inflammation (CD45), vascularity (CD31 and α-SMA), and hypoxia (pimonidazole). Results: Altering the order of therapies did not significantly affect BT474 cell proliferation in vitro (P>0.05). The interaction index calculations revealed additive effects of trastuzumab and radiation treatment in all three cell lines in vitro. In vivo results revealed significant differences in tumor response between mice treated with 5 and 10 Gy single agent radiation (P < 0.001); however, no difference was seen in the combination groups when trastuzumab was added to the radiation regimen (P=0.56), indicating a lower dose of radiation could be used without decreasing therapeutic efficacy. Histology results revealed increases in inflammation (CD45+) in mice receiving trastuzumab (P<0.05). Conclusions: Longitudinal evaluation of cell proliferation in vitro showed additive effects of combination therapy. In vivo results show a potential to achieve the same efficacy of treatment with reduced radiation when also administering trastuzumab. Further evaluation of tumor microenvironmental alterations during treatment could identify mechanisms of increased therapeutic efficacy in this regimen.

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“…Culture. HFS cells were cultured in DMEM, which contained 10% fetal bovine serum (FES) and 1% antibiotic (penicillin and streptomycin) [18]. The cultured cells were incubated in a CO 2 incubator (37 ∘ C, 95% air + 5% CO 2 ) [19].…”

Section: Hsf Cellsmentioning

confidence: 99%

Biological Evaluation of the Effect of Galvanic Coupling Intrabody Communication on Human Skin Fibroblast Cells

Shi

Gao

Cai

et al. 2017

Wireless Communications and Mobile Computing

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Intrabody communication (IBC) is an effective way to connect various kinds of wearable devices attached on or under the surface of the body, but it is important to quantitatively evaluate the biological effects of the IBC signal on the human body before its further application. The research described in this paper analyzed the responses of HSF (human skin fibroblast) cells exposed to IBC electrical signals. A galvanic coupling IBC signal transmitting system was designed to expose the experimental samples with different amplitudes (from 0 V to 6 V or 0 mA to 4 mA), different frequencies (from 10 kHz to 1 MHz), and different duration times (12 h and 24 h). The control groups were unexcited. Cell morphology and activity were evaluated with inverted microscope and MTT assays. The cell survival rates of all the experiment groups were in the range of 90% to 110%. Then, the data was analyzed by t-tests to assess whether there were statistically significant differences. The results showed that p values were greater than 0.05, so there were no significant differences between the experimental and control groups. Therefore, it can be concluded that the IBC signals do not have a significant effect on HSF cells.

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Different Cell Viability Assays Reveal Inconsistent Results After Bleomycin Electrotransfer In Vitro

Cited by 42 publications

References 47 publications

Discovery and identification of potential biomarkers for alcohol‐induced oxidative stress based on cellular metabolomics

Discovery and identification of potential biomarkers for alcohol‐induced oxidative stress based on cellular metabolomics

Quantifying the effects of combination trastuzumab and radiation therapy in human epidermal growth factor receptor 2 positive breast cancer

Biological Evaluation of the Effect of Galvanic Coupling Intrabody Communication on Human Skin Fibroblast Cells

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