Wearable interfaces are central to multiple healthcare and wellness strategies encompassing diet and nutrition, personalized health monitoring, and performance optimization.
A novel method based on atomic force microscopy (AFM) working in Ringing mode (RM) to distinguish between two similar human colon epithelial cancer cell lines that exhibit different degrees of neoplastic aggressiveness is reported on. The classification accuracy in identifying the cell line based on the images of a single cell can be as high as 94% (the area under the receiver operating characteristic [ROC] curve is 0.99). Comparing the accuracy using the RM and the regular imaging channels, it is seen that the RM channels are responsible for the high accuracy. The cells are also studied with a traditional AFM indentation method, which gives information about cell mechanics and the pericellular coat. Although a statistically significant difference between the two cell lines is also seen in the indentation method, it provides the accuracy of identifying the cell line at the single‐cell level less than 68% (the area under the ROC curve is 0.73). Thus, AFM cell imaging is substantially more accurate in identifying the cell phenotype than the traditional AFM indentation method. All the obtained cell data are collected on fixed cells and analyzed using machine learning methods. The biophysical reasons for the observed classification are discussed.
Hypoxia is a well-known component of many tumors and acts in an immunosuppressive manner through multiple distinct pathways. 18 F-FMISO PET imaging has been demonstrated to accurately quantify hypoxia in a clinical setting, although it has not been investigated for immunotherapy. Our work demonstrates that effective immunotherapy prevents tumor hypoxia and that uptake of 18 F-FMISO was predictive of subsequent changes in anatomical tumor size. We further demonstrate that PET imaging of hypoxia is correlated with phenotypic characteristics of inflammation through transcriptomics, immune cell spatial correlation, and direct measurement of secreted inflammatory proteins. The upregulation of damage associated molecular pattern signaling in responding tumors warranted investigation of the addition of the hypoxia targeted pro-drug to non-responding tumors in order to enhance checkpoint blockade efficacy. The addition of evofosfamide improved tumor oxygenation and response, providing rationale for immediate clinical investigation of both 18 F-FMISO PET imaging and combination evofosfamide therapy for improved cancer immunotherapy.Research.
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