Nippon Eiyo Shokuryo Gakkaishi (J. Jpn. Soc. Nutr. Food Sci.) 41, 305-308 (1988) New methods for vitamin Bi assay were devised using Euglena gracilis z and compared with HPLC analysis in the determination of vitamin contents of certain foods. 14C-Vitamin Bi dilution method; vitamin B1 was assayed on the basis of the 14C-vitamin Bi dilution effect by the addition of radio-inert vitamin B1 during vitamin B1 uptake by vitamin B1-limited Euglena cells (assayable range; 3-500ng). Vitamin B1 bioassay using vitamin B1-deficient Euglena cells; vitamin Bi was assayed by estimating the increase in cell number 24h after addition of vitamin Bi to the deficient cells (assayable range; 6-70 ng).Each of the assay systems showed high sensitivity and good reproducibility, as with HPLC.
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