Requirement and accumulation of inorganic ions in Euglena gracilis z.

宏倫, 越智; 成, 重岡; 義富, 飯塚; 文雄, 渡辺; 長久, 中野; 正三郎, 北岡

doi:10.4327/jsnfs.43.54

Cited by 1 publication

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“…This may be because when the E. gracilis cells were transferred to a fresh medium, adequate organic nutrients and inorganic ions induced the generation of intracellular components prior to cell multiplication. In detail, the proliferation rate of E. gracilis cells can be accelerated by the ions Mg 2+ , Ca 2+ , Mn 2+ , Cu 2+ , Co 2+ , and Ni 2+ in the medium 26 , and cell multiplication occurs slightly later than chloroplast multiplication. For E. gracilis cells, the number of chloroplasts in each cell is relatively stable, varying from 10 to 20 27 .…”

Section: Organic Nutrients and Biomass Accumulationmentioning

confidence: 95%

“…Although some inorganic metal ions are required for E. gracilis cell growth and are stabilized during biomass synthesis 26,30 , the organic supplies may be insufficient in the natural environment. Thus, E. gracilis cells have to grow photoautotrophically, and most of their biomass has to be produced by photosynthesis using carbon dioxide from the air as the carbon source 31,32 .…”

Section: Inorganic Ions and Cell Multiplicationmentioning

confidence: 99%

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Assessment of the electrical penetration of cell membranes using four-frequency impedance cytometry

et al. 2022

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The electrical penetration of the cell membrane is vital for determining the cell interior via impedance cytometry. Herein, we propose a method for determining the conductivity of the cell membrane through the tilting levels of impedance pulses. When electrical penetration occurs, a high-frequency current freely passes through the cell membrane; thus, the intracellular distribution can directly act on the high-frequency impedance pulses. Numerical simulation shows that an uneven intracellular component distribution can affect the tilting levels of impedance pulses, and the tilting levels start increasing when the cell membrane is electrically penetrated. Experimental evidence shows that higher detection frequencies (>7 MHz) lead to a wider distribution of the tilting levels of impedance pulses when measuring cell populations with four-frequency impedance cytometry. This finding allows us to determine that a detection frequency of 7 MHz is able to pass through the membrane of Euglena gracilis (E. gracilis) cells. Additionally, we provide a possible application of four-frequency impedance cytometry in the biomass monitoring of single E. gracilis cells. High-frequency impedance (≥7 MHz) can be applied to monitor these biomass changes, and low-frequency impedance (<7 MHz) can be applied to track the corresponding biovolume changes. Overall, this work demonstrates an easy determination method for the electrical penetration of the cell membrane, and the proposed platform is applicable for the multiparameter assessment of the cell state during cultivation.

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Section: Organic Nutrients and Biomass Accumulationmentioning

confidence: 95%