(2010). Brown spot of Indian mulberry (Morinda citrifolia L.) caused by Alternaria alternata. Jpn. J. Phytopathol. 76: 97-99.Leaf spots and brown mottled fruit were recorded on Indian mulberry (M. citrifolia L.; Rubiaceae) in Okinawa, Japan during 2007. An Alternaria species was isolated from the diseased leaves and fruits, and typical symptoms were reproduced after inoculation of wounded or intact leaves. The causal agent was identified as Alternaria alternata (Fr.) Keissler based on morphological characters and growth temperature. This report is the first of Alternaria spot of Indian mulberry caused by A. alternata.
To clarify the existence of latent infection and infection stages of the pathogens causing anthracnose on mangos grown in rainout shelters during bud formation, flowering, and the early stages of fruit set, we collected healthy-looking apical buds, flower buds, inflorescences and fruitlets and attempted to isolate the pathogens using an ethanol immersion treatment. We observed salmon-colored conidial masses on all organs sampled and isolated 34 isolates. Based on polymerase chain reaction analysis using species-specific primers, 23 isolates were identified as Colletotrichum gloeosporioides and the remaining 11 as C. acutatum; both were shown to be pathogenic to mango fruit (cv. Irwin). We generated a nitrate non-utilizing (nit) mutant of anthracnose fungus C. gloeosporioides isolate S754 (nit1) and used it in inoculation tests at various early stages of fruit set in an orchard to determine the time of infection. The nit1 isolate was recovered from lesions on fruit with latent infections that were inoculated at each stage: apical bud (inoculated in January), flower bud (February), inflorescence (April) and fruitlet (May). The isolate was recovered in greatest quantity from fruit inoculated at the fruitlet stage and in lowest quantity from fruit inoculated at the apical bud stage. These results show that these two species of Colletotrichum may both be present in healthy-looking apical buds, flower buds, inflorescences, and fruitlets and that infection during flowering, fruit set or fruit enlargement may remain latent within plant tissues for long periods, only causing lesions in the fruit peel after harvest.
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