“…Next, 4% sodium alginate solution was prepared using BPE, 250 mL of this solution was added to the Erlenmeyer flask (500 mL), and an equivalent amount of GYM liquid medium containing partial hyphae was then added, such that the total volume was adjusted to 500 mL. In accordance with the method of Taba et al ( 35 ), the simple mixed granular formulation (approximately 2 mm in diameter) was produced using this mixed solution. Four different formulations were produced using this method: liquid medium (GYM), BPE (stock solution, 10-fold dilution), hyphal bodies, and a mixture of BPE (stock solution, 10-fold dilution) and partial hyphae.…”