摘要:土壤宏转录组学是通过制备土壤 RNA 样本、RNA 测序、以及利用一系列生物信 息学方法和平台搭建来完成土壤微生物组的转录过程分析,提供关于基因表达和土壤微 生物组功能活性,从而获得微生物组关键代谢差异表征等信息的一门学科。关键点是针 对土壤 RNA 样本,表征特定条件下执行各个代谢过程的微生物活性特征,极大地规避 了因高通量 DNA 测序带来无法准确反映土壤微生物代谢活性的缺陷。本实验以两种类 型(酸性和碱性)湿地土壤样本为例,详述了利用市售 RNA 提取试剂盒进行的土壤宏 转录组样本制备流程,为准确评价土壤 RNA 样本制备提供参考,同时给出了宏转录组 数据分析流程,为从 RNA 水平分析土壤微生物表达活性提供思路。 关键词:土壤,宏转录组学,RNA,土壤微生物代谢活性 材料与试剂 1. 50 ml 离心管 (Thermo Fisher Scientific, USA) 2. 酚氯仿异戊醇 (配比 25:24:1, pH=8) 3. 琼脂糖 4. 电泳缓冲液 (TAE) 5. RNA 提取试剂盒 RNA Mini Kit (Qiagen, Hilden, Germany)
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.