Cold injury is one of the main problems in winter wheat production in the Huanghuaihai wheat region. Winter wheat grows rapidly at the jointing stage, and becomes sensitive to the drops in temperatures that frequently occur in spring. Low temperature during the jointing stage restricts the growth of winter wheat and directly affects grain yield. Therefore, correctly evaluating and improving the cold resistance of wheat varieties is very important for grain security. In this study, we evaluated the cold tolerance of 24 wheat varieties grown in the Huanghuaihai region. The wheat plants were grown in pots outdoors, and they were moved into an artificial climate chamber and subjected to a cold treatment at the jointing stage. After the cold treatment, we measured seven physiological indices in leaves; relative electrical conductivity, soluble sugars content, soluble protein content, free proline content, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and peroxidase (POD) activity. The results showed that the soluble protein content was lower in low鄄temperature鄄 treated plants than in the control plants (P<0.01) , while the other six indices showed higher values in the low鄄temperature鄄
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