A comparative study on the TBILI, DBILI, BUN of hybrid snakehead and its parents was conducted by Automatic Biochemical Analyzer. The results based on statistical analysis showed that the TBILI of hybrid snakehead was not significantly different from femail parent Channa maculata, while was significantly different from male parent Channa argus(P < 0.05), but the DBILI and BUN of hybrid snakehead were not significantly different from male parent Channa argus, while were significantly different from femail parent Channa maculata(P < 0.05), so it could be inferred that the inherited character of hybrid snakehead is the same with female parent, but is different from male parent refered to TBILI, while the inherited character of hybrid snakehead is the same with male parent, but is different from female parent refered to DBILI and BUN.