The aim of the research was to study some indices of morphofunctional state of lymphocytes in the peripheral blood under a complicated course of pregnancy in women of Khabarovsk. 96 women were examined in different trimesters of gestation. Two main groups depending on the type of complications were formed: the first group consisted of pregnant women with vaginitis, the second group had women whose pregnancy was complicated with clinical manifestations in the form of edemas, proteinuria and hypertensive disorders. The group of comparison included the women without complications. In the peripheral blood of the women under study with the method of high tech automated digital system of blood smear test VISION HEMA there was done a morphometric assessment of lymphocytes and with histochemical methods there was found a level of fermentation activity of succinate dehydrogenase (SDH), α-glycerophosphate dehydrogenase (α-GPDH) and lactate dehydrogenase (LDH). By morphometric studies of lymphocytes of the peripheral blood in pregnant women of the first group in the III trimester of gestation the indices of the square of the cell, of cytoplasm, cytoplasm brightness, the square of the nucleus and the brightness of the nucleus were 102.33±9.83 mсm², 36.84±4.88 mсm², 181.39±6.94 units of optical density, 65.69±6.87 mсm², 97.44±6.88 units of optical density, respectively (in the group of the comparison these data were 121.31±4.44 mсm²; 46.14±2.52 mсm²; 167.74±3.44 units of optical density; 75.33±2.66 mkm², 88.00±3.36 units of optical density, respectively). In the second group of women the same data in the same period of pregnancy corresponded to the following values: 128.44±4.89 mсm², 57.15±3.87 mсm², 173.53±4.65 units of optical density, 70.95±3.39 mсm² and 91.13±4.93 units of optical density (in the group of comparison there were the following data: 109.92±3.57 mсm², 39.63±1.53 mсm², 158.96±2.63 units of optical density, 75.37±3.23 mсm², 82.16±2.58 units of optical density, respectively). By the results of histochemical studies the level of activity of enzymes SDH, α-GPDH, LDH in the first group of pregnancy was 15.38±2.10, 10.72±0.58 and 20.53±2.36 pellet/cell, respectively. And just the other way round in pregnant women of the second group there was revealed a statistically reliable suppression of fermentation activity of these enzymes: 9.11±0.75 (p<0.05); 9.61±1.06 (p>0.05); 8.31±1.20 (p<0.05) pellet/cell in regard to the data in the group of comparison (12.84±1.21; 12.18±1.13 and 13.09±0.78 pellet/cell, respectively). Thus the changes of morphometric indices characterizing parameters of the cell, the nucleus and lymphocytes cytoplasm in a complex study with their fermentation activity can be extra diagnostic criteria of the formation of different complications during pregnancy. The obtained data allow to justify in-time application of medications for the correction of metabolic disorders of immune competent cells with the aim to decrease unfavorable perinatal outcomes.
Sixty patients at the age of 43 to 79 years with anxiodepressive symptoms with comorbid cardiovascular disease have been examined after admission to psychotherapeutic department. Patients have been divided into two groups. The first group including 30patients were treated by the tricyclic antidepressant amitriptyline with the average daily dose of 34.4±12.9mg. The 30 patients from the second group were treated by antidepressants of the selective serotonin reuptake inhibitors group. The sociodemographic characteristics in both groups did not differ significantly; the sever-ity of cardiovascular disease, additional somatic and other diseases were certainty lower in the first group than in the second (p<0.05). Examination of the patients with the use of Hamilton Depression Rating Scale and Hamilton Anxiety Rating Scale (HDRS and HARS) was carried out three times: at admission, in 1 week and in 4 weeks. At the time of admission, anxiety and depression in the first group were more expressed (p<0.05) than in the second due to frequent psychogeny. The treatment scheme included tranquilizers, neuroleptics, neurometabolic drugs, basic cardiotropic therapy and psychotherapy. After 4 weeks anxiodepressive symptoms were more than 50% reduced in both groups: 61% in the first group and 50.7% in the second group by the HDRC scale; 65.9% in the first group and 59.8% in the second group by the HARS scale. It has been shown that the small doses of antidepressant including amitriptyline involved in the complex treatment scheme were efficient and did not cause cardiotoxic effect
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.