Streptomycetes are known as the producers of various bioactive substances widely used in agriculture and many branches of industry. Different biotechnological methods and approaches are used to increased productivity of streptomycetes. In the present study, S. albus strains were subjected to mutagenesis associated with multistage selection and mutant strain with an increase of 3.5 times bacteriolytic enzyme complex production was obtained. Analysis of cell survival rate, antimicrobial and lytic activity as well as enzyme complex production in Streptomyces exposed to the various mutagen combinations (MNU, UV-irradiation, HNO 2 ) were carried out. Mutant strains were characterized by high bacteriolytic enzyme activity and significant antibiotic activity against Kocuria (Micrococcus) variants and Candida albicans. To estimate genomic changes caused by different mutagens, PCR with primers to short nucleotide repeats was performed and amplicons distinguishing parental and mutant strains were defined. The efficacy of the sequential mutagenic treatment for the hyperproducer obtaining as well as the relationship between the mutagen nature and the genomic variability has been shown. The results obtained in our research can be used and applied to the others Streptomyces species.
In this work the influence of experimental forms of biological product from Streptomyces albus UN44 on the growth processes of agricultural plants (wheat, peas, corn) was studied. The advantages of the preparation are demonstrated, which contains not only antibiotic and enzymatic complexes, synthesized by the producer, but also its biomass for the stimulation of seed germination processes, growth and development of the said plants seedlings. The proposed process description of biological product Streptofungin-Phyto production in two forms is the basis for engineering development and operating regulations. The preparation can be developed as a suspension with a cell titer of 109 CFU/cm3 in PET-containers (2 – 5 l) or as a dried mass in plastic bags (1 – 2 kg). The biological product can be recommended for use on the stage of pre-planting treatment of seeds by soaking, as well as deep root watering and irrigation. The remedy is safe for humans, animals and plants and can be used together with other microbial mass-based biofertilizers.
Background. The fight against the spread of infectious diseases creates the problem of resistance to pathogens and the most resistant of them – the propagators of nosocomial infections – are formed in hospitals because of a number of reasons. The solution of the problem lies in different areas, but the search of new effective means for the treatment of such diseases remains relevant right today. The shortest way to do this is to find the "pain points" of the pathogens themselves, i.e. the factors of their pathogenicity and resistance to which the action of novel antiseptics should be directed. Objective. We aimed to analyse and evaluate the main factors of pathogenicity and resistance of pathogens of nosocomial infections to determine modern approaches to the development of novel antimicrobials. Methods. Search and systematization of new scientific data and results concerning pathogenic factors of microbial pathogens that can be used as targets for the action of drugs. Results. Over the last 10–20 years, due to the development of new research methods in biology, it has become possible to clarify the features and additional conditions for the detection of pathogenic factors of nosocomial infections. Additional mechanisms of manifestation of resistance, adhesiveness, invasiveness, transmission of signs, secretion of toxins by pathogens are shownthat determines the general increase of their resistance to the action of currently used means. The general idea of creating antiseptics that will not increase the resistance of pathogens can now be implemented by using substances with multidirectional or indirect mechanisms of action that minimally affect the metabolism of the cell and significantly reduce its resistance and pathogenicity. Conclusions. Factors of pathogenicity of propagators of nosocomial infections and mechanisms of their implementation can be considered as the main targets for the action of novel antiseptics that will inhibit the spread of pathogens without increasing their resistance. The promising substances for such drugs, among other things, are bacteriophages and their modifications, enzybiotics, immunobiotics, autoinducer inhibitors, quorum sensing-system inhibitors, b-lactamase inhibitors and others. Some of these substances in combination with the new generation of antibiotics significantly enhance their effectiveness and together they are able to overcome the resistance of even multidrug-resistant pathogens.
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