In the present study, we have examined association between different polymorphic variants of metalloproteinases genes and clinical manifestations of bronchial asthma in children. We observed 103 patients including 42 children with an established diagnosis of asthma. Moreover, 61 persons were examined in the control group. All patients underwent genetic testing by allele-specific polymerase chain reaction. In particular, 320A>C polymorphic locus of ММР20 gene; Val275Ala ММР20, and -8202A>G gene ММР9 were analyzed.We have found that 30 patients (71.4% of total) had bronchial asthma of mild severity, 9 children (21.4%) exhibited moderate degree, and 3 patients (7%) had severe-grade disease. Homozygous C/C variant of the polymorphic ММР20 gene, 320A>C heterozygous variant of the ММР20 Val275Ala polymorphism, and heterozygous locus of -8202A>G ММР9 gene were found to be most frequent among the children with asthma. Generally, we have observed that the frequencies of the studied alleles and genotypes did not significantly differ berween the asthma patients and children from the control group (p < 0.05). However, in patients with GGgenotype of -8202A>G ММР9 polymorphism combined with homozygosity for the C allele of ММР20 320A>C, a more severe disease was observed, being combined with polyvalent sensitization and high total IgE levels in blood serum.In conclusion, frequencies of alleles and genotypes among patients with asthma did not show any statistically significant differences from the group of healthy children. The patients homozygous for G allele of ММР9 -8202A>G polymorphism gene and for the C allele ММР20 gene (320A>C) seem to be predisposed for a more severe clinical course of the disease.
Aim. The aim of the study was to reveal features of vegetative dysfunction syndrome in children with exacerbation of asthma.Materials and methods. 86 children with asthma of various severity, aged 6 to 18, were examined in this study. All patients underwent a comprehensive clinical and laboratory examination, which included anamnesis collection, physical examination, and laboratory tests, cardiointervalography study at rest and after clinoorthostatic sample. The received cardiointervalograms were processed using the software of the ANKAR-131 and Statistica 12.0 for Windows.Results. Statistically significant increase in heart rate [as well as indicators of the Mo and AMo (p=0.001)] were recorded in patients due to clinoorthostatic sample. Indicators of stress index in children in the period of exacerbation of the disease amounted to 119.36±17.93%/s×s. The vegetative balance was in equilibrium at rest (the index of vegetative equilibrium was within 156.48±21.30%/s), while the shift in the direction of the predominance of the sympathetic department was noted after a minor orthostatic load. The increase of corresponding values of the vegetative index and the adequacy index of the regulatory processes were recorded after the sample (95.56±5.89%/s). Our studies have shown that the more severe the disease, the more significant the changes in patients vegetative parameters.Conclusion. It was established that activation of the sympathetic regulation department and centralization of heart rate
Matrix metalloproteinases (MMP) play a special role in the pathogenesis of atopic dermatitis (AD). Therefore, the study of the features of genes responsible for the synthesis of MMP in children with AD is of great scientific and practical interest. Background. To study the role of polymorphic variants of matrix metalloproteinase genes (MMP 9 and MMP20) in the pathogenesis of AD in children. Materials and methods. Allelic variants of320A>C gene MMP20,837T>C gene MMP20, -8202 A>G gene MMP9 in children with AD were studied using the method of allele-specific polymerase chain reaction. The control group consisted of I and IIa the health groups patients of the corresponding sex and age. Results. The results of genetic studies showed that the incidence of alleles and genotypes in the polymorphisms 320A>C of the gene MMP20, 837T>C of the gene MMP20 in patients had no significant differences from the control group (p>0.05). It was established that the A/A-genotype of polymorphism -8202 A>G of the MMP9 gene, prevailed among children suffering from AD at a frequency of 69.2%, whereas in the group of healthy children the frequency of this genotype was 3 times lower (p=0.003). At the same time A/G-genotype (55.7%) was predominant in the control group, while G/G genotype was 2 times lower (21.3%). Thus the risk of AD increased by 7.55 times (OR=7.55 [95% Cl - 2.97-19.21; pG of the MMP9 gene, in particular the risk of developing of skin manifestations of allergy is increased by more than 7 times in A-allele homozygotes.
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