Ultraphonophoresis of eplire in combination with antibacterial therapy during experimental inflammation of uterine appendages activates fibroclasts and macrophages, prevents hyperplasia of connective ovary stroma and the development of fibrosclerotic alterations and adhesive process.Key Words: eplire; inflammation," ovaries," oviductsThe inflammatory diseases of uterine appendages are characterized by long-term relapsing course and the development of adhesions and fibrosclerotic alterations of the connective tissue in small pelvis in 35-37% women [8][9][10], which is the major cause of tuboperitoneal infertility, extrauterine pregnancy, and pelvic pain syndrome. The new drug preparation eplire, an extract of high-polar lipids of silt sulfide mud, was developed at Research Petrochemical Institute, Siberian Division of the Russian Academy of Sciences (Tomsk). It exhibits antioxidant, membrane-stabilizing, and pronounced antiinflammatory effects [1,7]. Beneficial therapeutic effect of eplire in experimental adnexitis (EA) was demonstrated [2].Our aim was to study the effect of eplire ultraphonophoresis (EUP) in combination with antibacterial therapy on fibrosclerotic and adhesive alterations in uterine appendages during EA. MATERIALS AND METHODSExperiments were carried out on 98 outbred female rats weighing 180-200 g divided into four groups. Intact rats served as the control (n=18), while in other rats EA was modeled [5]. To this end, the rats were narcotized with ether and Staphylococcus aureus culDepartment of Histology and Embryology, Department of Obstetrics and Gynecology, Siberian State Medical University, Tomsk ture (strain No. 209, two doses of 50 mln microbial bodies) was injected through insulin syringe into each uterine horn near oviducts. The test group 1 rats (n=25) received EUP for 10 days starting from day 5 of EA against the background antibacterial therapy (20,000 U/kg penicillin administered from day 3 of EA). Before physiotherapy the skin was depilated. Eplire (0.5 ml, 1% oil solution) was applied to the skin immediately prior to EUP. Group 2 rats (n=30) received antibacterial therapy alone. Group 3 rats (n=25) was subjected to a 10-day ultrasound therapy starting from day 5 of EA against the background antibacterial therapy. Ultrasound therapy and EUP were performed with an UZT-31F apparatus with due account for species-specific features of experimental animals [6]. Both fields corresponding to projections of uterine appendages were irradiated with ultrasound according to a labile method in a pulse mode (4 m/ sec, 0.05 W/cm z beam power, 0.3 sec pulse duration, 3-4 rain exposure).The rats were decapitated during diestrus (colpo, cytological control) on days 5, 10, 15, 30, 75, and 120 of EA. For electron microscopy, the oviductal and ovarian specimens were fixed in 2.5% glutaraldehyde in cacodylate buffer (pH 7.2), postfixed in 1% osmium tetroxide, and embedded in Araldite. The specimens were cut on LKB-III ultratome. Semithin sections were stained with toluidine blue, and ultrathin sections were...
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