The purpose of the research is determination of optimal muscle tissue areas for confirmation of diagnosis in the lynx by trichinelloscopy.Materials and methods. The distribution of Trichinella sp. larvae were studied in spontaneously infected lynxes by the trichinelloscopy method when examining 72 sections in a compressorium. In addition to calculating the total number of isolated larvae and the percentage in muscles, we recalculated per 1 g of animal muscles and performed morphometry of the area and perimeter of Trichinella sp. capsules from various muscles, and calculated the ratio of the capsule length and width, and the M value (arithmetic mean): the area of the capsule and its perimeter. We determined the percentage ratio of the larva size to the capsule and the position in the myosymplast using the Vision Bio system (Epi 2014). The results were statistically processed using the Microsoft Excel software package. The larvae were photographed directly from the screen with digital signal processed by the Vision Bio system (Epi 2014).Results and discussion. It has been found that the capsules in the muscles are more often rounded (90% of all Trichinella sp. have a length-to-width ratio of 1.1 : 1). However, there are also oval capsules, the largest number of which was recorded in the masticatory muscles (33% of the total capsules in the masticatory muscles). When studying the selective expansion of larvae, it was found that the masticatory muscles, tongue, diaphragm and gastrocnemius muscles were the most populated. Conclusion. We identified high prevalence and intensity of infection in the lynx, which indicates not only the epizootic significance of the lynx in the spread of trichinellosis in the Kirov Region, but also its epidemiological significance as a source of infection. Due to the fact that the meat of this animal is eaten and there is a risk of human infection, it is necessary to examine the carcasses of these animals for trichinellosis by the compressor trichinelloscopy method or by the peptolysis method.
Trichinellosis is the most dangerous zoonosis of badger. Contamination of Meles meles and Meles leucurus an T. spiralis of Kirov area is investigated. It has been studied badger meat naturally infeсted with T. spiralis of the Кirov region. Trichinella larvae were found in all investigated slides of diafragm. Meles as a source of infection play the leading role in the synantropic infection. Meth-ods of trichinelloscopy can be used for postmortal-diagnostics of trichinellosis is aimed at detection of sources of infection and prevention of helminth zoonosis in human.
Background Trichinosis is a parasitic disease caused by ingestion larvae of Trichinella spiralis (TS). In Russia trichinosis of pigs is widely spreaded. Invasion occurs when human or animal eat contaminated meat. TS is adapted to many hosts, and due to its wide spread, there has always been a demand of safe alternatives for this infection. A lot of information has been gathered about sharp decreasing of quantity of trichinae in muscles at repeated invasion of predators. We took a chance of applying antigen to TS as a vaccine, however, rate of immunogenetic effect wasn't high. Therefore, in an experimental work, we decided to inject vaccine and immunomodulators together at the same time. Material and Methods We subjected rats infected with TS to conventional treatment (vaccine) along with Cina 6cH. There were five groups as follows: The first group was injected antigen alone and no immunomodulators; the second group was injected antigen and Ronkoleikin; the third group was injected antigen and Sodium nucleinate, the fourth group was injected antigen and Cina C6cH; and the fifth group was a control group, which was injected neither antigen nor immunomodulators. After 21 days of vaccination (or giving homeopathic medicine) rats were contaminated TS in a dose of 200germs/animal. Results The rats were euthanized after two months. They we then analysed for stomach contents and quantity of worms in muscles. The highest number of worms were found in fifth group (no antigen and no immunomodulators) (more than 2600 worms/animal. The quantity of worms in the second group (injection of antigen and Ronkoleikin) was in four times less than in the control group. The most efficient substance was Ronkoleikin (900+160 worms/animal); Sodium nucleinate (1100+100 worms/per animal); Cina C6cH (1400+50 worms/animal). Conclusion Cina C6cH along with and others immunomodulators was able to exhibit protective effect against experimental trichinosis. Further research with rigorous designs is needed to explore the role of Cina in Trichinella spiralis.
Результаты и обсуждение. В РФ зарегистрирован лейшманиоз в Дагестане, Крыму и т. д. В настоящее время лейшманиоз собак широко распространен в полуостровной и островной Италии. Спорадические вспышки встречаются к востоку от Апенин, в центральной Италии и в некоторых районах северного Пьемонта и Валле-д'Аоста, а также в провинциях Верона, Брешиа и Римини. Много случаев зарегистрировано в Тоскане. Борьба с лейшманиозом основана главным образом на выявлении и лечении инфицированных собак в сочетании с контролем переносчиков и контролем над резервуарами животных. Более 30% образцов были положительными в Италии и 0,7% образцов были положительными на юге РФ. Более 80% сывороток, прореагировавших с лейшманиозным антигеном (1/160 и более), реагировали в ИФА с трихинеллезным и токсокарозным антигенами. Полученные данные о спорадических случаях на юге РФ свидетельствуют о необходимости проведения эпидемиологических, эпизоотологических и энтомологических обследований в указанных областях и разработки системы эпиднадзора за лейшманиозом, охватывающей весь комплекс противоэпидемических мер.
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