In this article, a series of silver-containing dressings are prepared by metal-vapor synthesis (MVS), and their antibacterial properties are investigated. The antibacterial activity of the dressings containing silver nanoparticles (AgNPs) against some Gram-positive, and Gram-negative microorganisms (Staphylococcus aureus, Staphylococcus haemolyticus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Moraxella spp.) has been determined. Based on the plasmon resonance frequency of these nanoparticles, the frequency of laser irradiation of the dressing was chosen. The gauze bandage examined showed pronounced antibacterial properties, especially to Staphylococcus aureus strain. When 470 nm laser radiation, with a power of 5 mW, was applied for 5 min, 4 h after inoculating the Petri dish, and placing a bandage containing silver nanoparticles on it, the antibacterial effect of the latter significantly increased—both against Gram-positive and Gram-negative microorganisms. The structure and chemical composition of the silver-containing nanocomposite were studied by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and extended X-ray absorption fine structure (EXAFS). The synthesized AgNPs demonstrate narrow and monomodal particle size distribution with an average size of 1.75 nm. Atoms of metal in Ag/bandage system are mainly in Ag0 state, and the oxidized atoms are in the form of Ag-Ag-O groups.
1 УЗ «Гродненская областная клиническая больница», Гродно, Беларусь 2 УО «Гродненский государственный медицинский университет», Гродно, Беларусь Введение. Прогресс в рентгеноангиохирургии позволяет пересмотреть традиционные подходы в лечении ишемического инсульта вследствие тромбоза в бассейне внутренней сонной артерии (ВСА). Цель исследования. Изучить ранние и отдаленные результаты селективного тромболизиса в бассейне ВСА при его остром тромбозе. Материал и методы. Обследованы 13 пациентов в возрасте от 40 до 64 лет. Селективный тромболизис осуществлялся тканевым активатором плазминогена Actilizae -Boehring Ingelheim.Результаты. К моменту завершения раннего послеоперационного периода 10 пациентов выписаны на амбулаторное лечение, трое умерли от прогрессирования основного заболевания. При обследовании в сроки от 1 года до 5 лет в 9 случаях отмечено восстановление двигательной активности и речи.Выводы. Улучшение результатов селективного тромболизиса в бассейне ВСА требует сокращения времени от начала тромбоза до поступления пациента в ангиографический кабинет.Ключевые слова: внутренняя сонная артерия, тромбоз, селективный тромболизис.
Comparison of the healing rates of different wound types using natural and synthetic polymers, containing silver nanoparticles. Experimental purulent and aseptic wounds are created on white rats. In the course of treatment, dressings are performed daily, including photographing the wound and applying various types of nanocomposite polymer materials. Cotton gauze, which is a polysaccharide, is taken as a natural polymer. The synthetic polymer is a nonwoven hydro‐bonded polypropylene material produced by extrusion of polypropylene filaments. Silver nanoparticles used in the study are obtained by the metal‐steam synthesis method. It is found that in all animals with aseptic or purulent created wound in the first 4 days of the experiment, an increase in the area of the wound is observed with respect to the initial one, much less pronounced in the presence of silver nanoparticles in the material. On the following days of treatment, up to complete healing, the decrease in the wound area in relation to the initial one in the experimental group (with silver nanoparticles) occurs more intensively than in the control group of animals (p < 0.05). Both natural and synthetic polymeric materials coated with silver nanoparticles synthesized by metal‐steam synthesis method accelerate the healing of both experimental aseptic and purulent wounds. However, the rate of wound healing when using natural or synthetic materials differs depending on the type of wound and the presence of microbial contamination, which should be taken into account when creating a new generation of dressings.
A quantitative assessment of the antibacterial effect of silver nanoparticles on polyantibiotic-resistant grampositive and gram-negative microorganisms was carried out. Silver nanoparticles were synthesized by the environmentally friendly metal-steam synthesis method. The size and electronic state of nanoparticles were investigated by transmission electron and X-ray photoelectron spectroscopy. The antibacterial properties of nanomaterials were assessed on two clinical pathogenic strains of gram-positive and four strains of gram-negative microorganisms. The typing and assessment of bacterial resistance to antibiotics were carried out on a microbiological analyzer. The antibacterial effect of nanoparticles was quantitatively assessed using the dilution method and the determination of the minimum inhibitory and minimum bactericidal concentrations.It was found that the studied silver nanoparticles have sizes in the range from 5 to 24 nm with an average diameter of 10.8 nm. It was shown that all clinical strains of microorganisms used in the study are characterized by multiple antibacterial resistance; the percentage of their antibiotic resistance ranges from 12.5 to 93.3 %. It was found that for the studied microorganism, the values of the minimum inhibitory concentration (MIC) are in the range from 7.81 to 31.25 μg/ml, and the minimum bactericidal concentration (MBC) is in the range from 31.25 to 62.50 μg/ml. The obtained MIC and MBC data can be used to create promising antimicrobial drugs and medical next generation devices.
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