ConclusionsOur data show that IFN-gÀ/À mice are resistant to the development of AA, pointing toward an important role of IFN-g in the pathogenesis of AA. The absence of a perifollicular lymphocytic infiltrate in the skin and the reduced expression of T cell activation markers and costimulatory molecules in skin-draining lymph nodes demonstrate that T cells are not activated in the lymph nodes of IFN-gÀ/À mice. Therefore, we conclude that IFN-g is involved in T cell activation in AA pathogenesis of C3H/ HeJ mice.A pathogenetic role for IL-2 in AA could also be demonstrated, but the cogency of our data is limited because only heterozygous IL-2 þ /À mice were available for these experiments. But Hollä nder et al (Science 1998) demonstrated that IL-2 expression is reduced 50% in IL-2 þ /À mice compared with IL-2 þ / þ mice. Our data show that the frequency of AA development is significantly reduced in IL-2 þ /À mice. Therefore, we conclude that the magnitude of IL-2 expression influences the frequency of AA development. FACS analysis and immunohistochemistry show that AA resistance of half of the IL-2 þ /À mice is related to a reduced T cell activation in the lymph nodes and an absence of perifollicular infiltrates of CD4 þ and CD8 þ cells in the skin. These data suggest that AA development in the C3H/HeJ mouse model depends on the level of IL-2 expression and on subsequent T cell activation in the lymph nodes.Taken together, our results show that the type-1 cytokines IFN-g and IL-2 are involved in T cell activation in the development of AA in the C3H/HeJ mouse model. Methods and ResultsAfter experimental induction of AA by skin grafting, 90% of normal C3H/HeJ mice (control) developed AA, whereas none of the IFN-gÀ/À mice and only 47% of IL-2 þ /À mice developed AA.Immunohistochemistry of skin sections showed dense peri-and intrafollicular infiltrates of CD4 þ and CD8 þ cells in control mice with AA, whereas perifollicular infiltrates were absent in IFN-gÀ/À and IL-2 þ /À mice without AA.FACS analysis of skin-draining lymph node cells and skininfiltrating leukocytes of IFN-gÀ/À mice revealed a significant reduction of the T cell activation markers and of co-stimulatory molecules. In IL-2 þ /À mice the expansion of CD4 þ T cells was reduced and the upregulation of activation markers and costimulatory molecules was impaired.
The article deals with the clinical aspects of inflammatory manifestations of dermatoses at an early age. It presents the results of the use of a non-steroidal anti-inflammatory drug based on activated pyrithione zinc
В статье приводятся обзорные данные относительно применения в клинической практике малоизвестного, но полезного в клинической практике а лгоритма дерматоскопической диагностики акральной лентигинозной меланомы кожи. Алгоритм BRAAFF является одним из инструментов принятия решений при анализе дерматоскопического изображения, получаемого при меланомах акральных локализаций. Ключевые слова: дерматоскопия, акральная меланома, алгоритм BRAAFF. Для цитирования: Жучков М.В., Шарова Н.М., Большакова Е.Е., Сонин Д.Б. Дерматоскопический алгоритм BRAAFF в диагностике акральной меланомы. Consilium Medicum. 2020; 22 (7): 38–41. DOI: 10.26442/20751753.2020.7.200305
The article deals with the features of skin structure and functions in infants and the causes of diaper dermatitis. It determined the priority of preventive measures and the choice of optimal agents for topical application that meet international standards. The requirements for topical agents used in infants for prevention of DD are described in detail, according to the international opinion of the experts group.
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