SummaryBackgroundChronic obstructive pulmonary disease (COPD) is an inflammatory disease associated with reactive oxygen species (ROS) production. The aim of this study was to evaluate the effect of Hypoxen® treatment and the effect of HyFnC60 on ROS production in patients’ blood.Material/MethodsROS production in blood was estimated using chemiluminescence (CL) measurement with CL-amplifiers: luminol (LM), LM + zymosan (ZM) or lucigenin (LC) in the presence or absence of hydrated fullerenes (HyFnC60) added to blood in low concentrations.ResultsIn all the patients with COPD in remission phase with Hypoxen® prescription, the LM-dependent CL (LM-CL) with ZM and LC-enhanced CL (LC-CL) decreased after the treatment. Parameters of CL and effects of HyFnC60 upon them depended on blood state. Addition of HyFnC60 to blood decreased data scattering and helped to improve discrimination between different groups of patients. Using the discriminator analysis, we found the most important time-points in the kinetic curves of CL for classification of patients into groups (eg, COPD patients before and after treatment with Hypoxen®; patients’ blood with different sensitivity to HyFnC60 concentration).ConclusionsMonitoring of CL of non-diluted whole blood in COPD patients can be used for the estimation of the Hypoxen® efficiency in complex therapy. Addition of HyFnC60 to blood increases sensitivity of the method.
Hydrated fullerene C60 (HyFnC60) is a symmetrical molecule of C60 Buckminster fullerene surrounded by a water shell obtained by an original technique. It can act as an antioxidant or a pro-oxidant in various biological objects, depending on the conditions. In this study we added HyFnC60 at a range of concentrations obtained by a method of serial dilutions with vigorous shaking at each step to whole undiluted blood of healthy donors and hospital patients with COPD and examined lucigenin-enhanced blood chemiluminescence. We have found that HyFnC60 at concentrations of 2.5 10 -6 M, 2.5 10 -7 M, 2.5 10 -17 M, and 2.5 10 -19 M increased lucigenindependent chemiluminescence in heathy donors' blood while in blood of patients with COPD it had an opposite effect. This can be interpreted as in healthy donors' blood reactive oxygen species (ROS) generation is enhanced by HyFnC60 while in patients with a chronic inflammatory disease with already increased ROS generation it is attenuated. This indicates that HyFnC60 preparations even in ultra-high dilutions may play the role of a tuner of the processes with ROS participation. Probable reasons of such action of HyFnC60 on human blood even in ultra-high dilutions are discussed.
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