Changes in chemiluminescence of whole blood of COPD patients treated with Hypoxen® and effects of C60 fullerenes on blood chemiluminescence

Новиков, К.Н.; Бердникова, Н. Г.; Новиков, А.К.; Lyusina, O.Y.; Muhitova, Olga G.; Yablonskaya, Olga I.; Minh, Ha Do; Воейков, В.Л.

doi:10.12659/msm.882460

Cited by 9 publications

(12 citation statements)

References 12 publications

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“…Hypoxen ® treatment of COPD patients resulted in signi icant alternation of LM-ZM-dependent and LC-dependent CL of whole blood of patients. This is consistent with the claimed strong antioxidant activity of Hypoxen ® [9].…”

Section: Resultssupporting

confidence: 91%

“…We obtained the dependence of LM and lucigenin (LC) ampli ied CL of a healthy donor's blood in the upon them depended on blood state. Using the discriminator analysis we found the most important time points in the kinetic curves of CL for classi ication patients into groups (e.g., COPD patients before and after treatment with Hypoxen ® ) [9].…”

Section: Chronic Obstructive Pulmonary Disease (Copd) and Patients' Bmentioning

confidence: 99%

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Peculiarities of photon emisson of whole non-diluted human blood obtained from healthy donors and patients with some diseases

N¹

2018

J Pulmonol Respir Res

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Blood plays an important role in oxygen absorption and its transfer to organs and tissues in vertebrates, as well as in a number of invertebrate species. Numerous interactions between cellular and non-cellular blood components constantly occur. A special role in these interactions belongs to erythrocytes and leukocytes, between which oxygen is constantly exchanged and activated, which we showed directly in whole blood. Blood is a liquid tissue, which is a complex cooperative system and has many inherent functions and the most important one is the ability to maintain the homeostasis of the body. Our experience has shown that despite its high optical density, undiluted blood of humans and animals can be a source of radiation due to the transformation of the energy of electron-excited (EEE) states and secondary processes occurring in the whole blood system. Parameters of this radiation -ultra-weak photons emission (UWPE) from blood -depend upon its physiological properties and refl ect the physiological state of a donor. Analysis of UWPE from non-diluted blood is a simple and sensitive method that allows to monitor the course of treatment of a patient. In spite of high opacity of nondiluted blood it may be a strong source of UWPE both in the presence and absence of UWPE enhancers. Analysis of patterns of UWPE from blood reveals its highly non-linear, stable non-equilibrium and cooperative properties. Characteristic of a living system.

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Section: Resultssupporting

confidence: 91%

Section: Chronic Obstructive Pulmonary Disease (Copd) and Patients' Bmentioning

confidence: 99%

Peculiarities of photon emisson of whole non-diluted human blood obtained from healthy donors and patients with some diseases

N¹

2018

J Pulmonol Respir Res

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“…Additional experiments under similar conditions should be performed to confirm this suggestion. Previously [61,62,63,64], the biological activity of hydrated C 60 fullerene was studied and its activity was attributed to specific structure of hydrated shell of the fullerene.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Activity and Toxicity of Fullerenols via Bioluminescence Signaling: Role of Oxygen Substituents

Kovel

Sachkova

Vnukova

et al. 2019

IJMS

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Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, a specific allotropic form of carbon, bioactive compounds, and perspective basis for drug development. Our paper analyzes the antioxidant activity and toxicity of a series of fullerenols with different number of oxygen substituents. Two groups of fullerenols were under investigation: (1) C60Oy(OH)x, C60,70Oy(OH)x, where x+y = 24–28 and (2) C60,70Oy(OH)x, Fe0,5C60Oy(OH)x, Gd@C82Oy(OH)x, where x+y = 40–42. Bioluminescent cellular and enzymatic assays (luminous marine bacteria and their enzymatic reactions, respectively) were applied to monitor toxicity in the model fullerenol solutions and bioluminescence was applied as a signaling physiological parameter. The inhibiting concentrations of the fullerenols were determined, revealing the fullerenols’ toxic effects. Antioxidant fullerenol’ ability was studied in solutions of model oxidizer, 1,4-benzoquinone, and detoxification coefficients of general and oxidative types (DGT and DOxT) were calculated. All fullerenols produced toxic effect at high concentrations (>0.01 g L−1), while their antioxidant activity was demonstrated at low and ultralow concentrations (<0.001 g L−1). Quantitative toxic and antioxidant characteristics of the fullerenols (effective concentrations, concentration ranges, DGT, and DOxT) were found to depend on the number of oxygen substituents. Lower toxicity and higher antioxidant activity were determined in solutions of fullerenols with fewer oxygen substituents (x+y = 24–28). The differences in fullerenol properties were attributed to their catalytic activity due to reversible electron acceptance, radical trapping, and balance of reactive oxygen species in aqueous solutions. The results provide pharmaceutical sciences with a basis for selection of carbon nanoparticles with appropriate toxic and antioxidant characteristics. Based on the results, we recommend, to reduce the toxicity of prospective endohedral gadolinium-fullerenol preparations Gd@C82Oy(OH)x, decreasing the number of oxygen groups to x+y = 24–28. The potential of bioluminescence methods to compare toxic and antioxidant characteristics of carbon nanostructures were demonstrated.

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“…Transient or long-term administration of the p38α inhibitor SB681323 significantly decreased the levels of p-HSP27 and TNF-α in whole blood and sputum neutrophils, indicating a remarkable reduction of airway inflammation [ 17 , 42 – 44 ] in patients with COPD. These studies suggest the pivotal role of p38 MAPK in the induction of airway inflammation in COPD patients, probably through CXCL8 signaling [ 6 , 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Activity of sputum p38 MAPK is correlated with airway inflammation and reduced FEV1 in COPD patients

et al. 2013

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BackgroundInflammation and remodeling of the small airways are major determinants of the progression and severity of COPD. The present study explored the correlation between sputum p38 mitogen-activated protein kinase (MAPK) activity and airway inflammation and reduction of lung function in the patients with chronic obstructive pulmonary disease (COPD).Material/MethodsSputum samples were collected from 48 COPD patients and 12 healthy persons. Sputum p38 MAPK activity was measured by Western blotting and sputum levels of CXCL8 and neutrophil, and lung function was measured. The correlation between p38MAPK activity and airway inflammation and reduction of lung function was analyzed.ResultOur results showed the significantly increased expression of phospho-p38 MAPK and CXCL8 in the sputum samples of the COPD patients. The p38 MAPK activity was remarkably correlated with the CXCL8 level and neutrophils infiltration in the airway, and the decline of lung function in the COPD patients.ConclusionsThese findings suggest the pivotal role of p38 MAPK in the airway inflammation of COPD patients. We propose p38 MAPK as a potential target for the treatment of COPD.

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Changes in chemiluminescence of whole blood of COPD patients treated with Hypoxen® and effects of C60 fullerenes on blood chemiluminescence

Cited by 9 publications

References 12 publications

Peculiarities of photon emisson of whole non-diluted human blood obtained from healthy donors and patients with some diseases

Peculiarities of photon emisson of whole non-diluted human blood obtained from healthy donors and patients with some diseases

Antioxidant Activity and Toxicity of Fullerenols via Bioluminescence Signaling: Role of Oxygen Substituents

Activity of sputum p38 MAPK is correlated with airway inflammation and reduced FEV1 in COPD patients

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