We have determined the averaged absorption spectra in the near UV and visible regions of the electromagnetic spectrum for aqueous solutions of hemolyzed blood, plasma, and serum from 100 donors and 95 patients with different diagnoses and different severity of their conditions. From the averaged absorption spectra, we calculated the color characteristics of the hemolyzed blood, plasma, and serum from the donors and patients by the standard CIE procedure. The blood is considered as a single, indivisible light-absorbing system in studying complex biological specimens.Introduction. Colorimetric studies of blood are actively used in medicine [1], criminal law, [2], and the food industry [3,4]. In medical practice, colorimetric methods are used to determine the hemoglobin concentration in the blood of a patient (the color index) [5]. Today a rather exact (±1%) cyanomethemoglobin photometric method is used everywhere, in which cyanomethemoglobin is determined at a wavelength of 540 nm after preparation of a working solution of the blood in Drabkin reagent. Various modifications of this method do not change its essential physical nature [6]. Furthermore, spectral analysis in the visible region has been used to determine oxyhemoglobin and other hemoglobin-containing compounds from the absorption spectra of blood and its solutions [6][7][8][9]. Despite this, the quantitative colorimetric characteristics of blood have not been studied before.The aim of this work was to study the color characteristics of hemolyzed blood, plasma, and serum from donors in the visible range of the absorption spectra by standard CIE methods (International Commission on Illumination, 1964).The basic color characteristics (lightness and chromaticity coordinates) determine the position of the color of the specimen in an arbitrary color space, and are found by the CIE method [10,11].The familiar spectrophotometric method for color measurements involves measuring the spectral power distribution of the radiation followed by calculation of the color coordinates by multiplying the determined spectral power distribution function times the three color-matching functions, and then integrating the products. For the spectral power distribution function of the source E(λ), the spectral transmittance function τ(λ), and x(λ), y(λ), z(λ) (the color-matching functions), the color coordinates X, Y, Z are determined by integration over the wavelength range for visible radiation 380-760 nm. In practice, integration is replaced by summation over the interval dλ (from 5 to 10 nm), since the spectral functions under the integral sign are usually not easily integrated:
The authors determined a connection between change of functional state of human with liver cirrhosis and color characteristics of blood plasma. Features of color characteristics of blood plasma of patients with liver cirrhosis as compared with those of healthy people were determined by colorimetric method, based on absorption electromagnetic radiation in visible area. The average chromaticity coordinates in the XYZ system for patients with liver cirrhosis are x = 0.352 ± 0.006, y = 0.356 ± 0.005 as compared with healthy people chromaticity coordinates x = 0.32 ± 0.001, y = 0.32 ± 0.002.
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