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Fiber flax is an important source of natural fiber and a comprehensive model for the plant fiber biogenesis studies. Cellulose-synthase (CesA) and cytoskeletal genes are known to be important for the cell wall biogenesis in general and for the biogenesis of flax fibers in particular. Currently, knowledge about activity of these genes during the plant growth is limited. In this study, we have investigated flax fiber biogenesis by measuring expression of CesA and cytoskeletal genes at two stages of the flax development (seedlings and stems at the rapid growth stage) in several flax subspecies (elongatum, mediterraneum, crepitans). RT-qPCR has been used to quantify the expression of LusСesA1, LusСesA4, LusСesA7, LusСesA6, Actin, and α-Tubulin genes in plant samples. We report that CesA genes responsible for the secondary cell wall synthesis (LusCesA4, LusCesA7) have different expression pattern compared with CesA genes responsible for the primary cell wall synthesis (LusCesA1, LusCesA6): an average expression of LusCesA4 and LusCesA7 genes is relatively high in seedlings and further increases in stems at the rapid growth stage, whereas an average expression of LusCesA1 and LusCesA6 genes decreases. Interestingly, LusCesA1 is the only studied gene with different expression dynamics between the flax subspecies: its expression decreases by 5.2-10.7 folds in elongatum and mediterraneum but does not change in crepitans subspecies when the rapid growth stage and seedlings are compared. The expression of cytoskeleton genes (coding actin and α-tubulin) is relatively stable and significantly higher than the expression of cellulose-synthase genes in all the studied samples.

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In order to use marker-assisted selection (MAS) methods in the development of hybrids and tomato varieties resistant to late blight, the efficiency of molecular markers to the alleles resistant to Phytophthora infestans known in the literature was assessed. High efficiency in the identification of homozygous and heterozygous forms using CAPS dTG63 (Hinf1) and SCAR NCLB-9-6678 markers to Ph2 and Ph3 alleles was established respectively. DNA extraction techniques, reaction mixture compositions, as well as amplification and visualization result modes were adapted. The presence of the Ph3 allele in F1-standard Adapt and Alamina hybrids, and F1 Azart, Agenchik, Dzivosny, and Black Brilliant hybrids included in the Register of Varieties of the Republic of Belarus, as well as in the breeding lines of the collections possessed by the Institute of Genetics and Cytology, NAS of Belarus, and the Belarusian State Agricultural Academy was demonstrated.

2 Белорусский государственный университет, Минск, Республика Беларусь ДИФФЕРЕНЦИАЛЬНАЯ ЭКСПРЕССИЯ ГЕНОВ УЗКОЛИСТНОГО И ЖЕЛТОГО ЛЮПИНОВ ПРИ ИНФИЦИРОВАНИИ ИЗОЛЯТАМИ FUSARIUM Аннотация. Методом SRAP-анализа кДНК изучена дифференциальная экспрессия генов у проростков узколистного и желтого люпинов, обработанных различными изолятами Fusarium. В результате выявлены ПЦР-фрагменты, присутствие которых в профилях коррелирует с невосприимчивостью проростков к патогену. Соответствующие им генетические детерминанты, вероятно, участвуют в обеспечении устойчивости (толерантности) растений люпина к фузариозу. Ключевые слова: люпин узколистный, люпин желтый, кДНК, SRAP-типирование Для цитирования: Дифференциальная экспрессия генов узколистного и желтого люпинов при инфицировании изолятами Fusarium / Е. Н. Сысолятин [и др.] // Докл. Abstract.In order to assess the differential expression of genes, seedlings of narrow-leaved and yellow lupine treated with different Fusarium isolates were studied by the method of SRAP-analysis. As a result, PCR fragments correlated with tolerance to infection were found. The corresponding genetic determinants are likely involved in the resistance (tolerance) control of lupine plants to Fusarium.Keywords: narrow-leafed lupin, yellow lupin, cDNA, SRAP genotyping For citation: Sysoliatin E. N., Anokhina V. S., Anisimova N. V., Babak O. G., kilchevsky A. V. Differential gene expression in Fusarium-treated seedlings of narrow-leaved and yellow lupine. Doklady Natsional'noi akademii nauk Belarusi = Введение. Люпины узколистный и желтый являются ценным источником растительного белка для кормопроизводства и представляют значимую альтернативу сое в европейских странах. В соответствии с действующей в Беларуси программой обеспечения животноводческой отрасли сельского хозяйства собственным растительным белком посевные площади под зернобобовыми культурами в последние годы растут (170 % к уровню 2018 г.). Однако расширение посевов люпина осложняется существующей угрозой потерь урожая вследствие инфекционных болезней. Серьезный экономический ущерб возделыванию люпина наносят грибные инфекции,

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