Cellulose synthase genes that control the fiber formation of flax (Linum usitatissimum L.)

Galinousky, Dmitry; Анисимова, Н. В.; Raiski, Andrei; Леонтьев, Виктор Николаевич; Титок, В. В.; Хотылева, Л. В.

doi:10.1134/s1022795414010050

Cited by 9 publications

(9 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly, the proposed CESA7B protein lacks an N‐terminal Zn domain and is most likely a CSL protein, rather than a true CESA. These observations seem to show that the flax genome only contains a single CESA7 gene as indicated in another recent paper (Galinousky et al ., ). Taken together, our results suggest that the flax genome contains 15 CESA genes (Figure ).…”

Section: Resultsmentioning

confidence: 97%

“…Given the cellulose-rich nature of the flax bast fibre cell wall and the importance of this polymer in determining fibre performance and quality, there are surprisingly only few reports on CESA genes in this species (Galinousky et al, 2014;Mokshina et al, 2014). The practical interest of such an investigation for flax is underlined by a recent study demonstrating that a functional relationship exists between CESA structures, cellulose crystallinity and saccharification efficiency in Arabidopsis (Harris et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Functional analyses of cellulose synthase genes in flax (Linum usitatissimum) by virus‐induced gene silencing

Chantreau

Chabbert

Billiard

et al. 2015

Plant Biotechnology Journal

View full text Add to dashboard Cite

SummaryFlax (Linum usitatissimum) bast fibres are located in the stem cortex where they play an important role in mechanical support. They contain high amounts of cellulose and so are used for linen textiles and in the composite industry. In this study, we screened the annotated flax genome and identified 14 distinct cellulose synthase (CESA) genes using orthologous sequences previously identified. Transcriptomics of 'primary cell wall' and 'secondary cell wall' flax CESA genes showed that some were preferentially expressed in different organs and stem tissues providing clues as to their biological role(s) in planta. The development for the first time in flax of a virus-induced gene silencing (VIGS) approach was used to functionally evaluate the biological role of different CESA genes in stem tissues. Quantification of transcript accumulation showed that in many cases, silencing not only affected targeted CESA clades, but also had an impact on other CESA genes. Whatever the targeted clade, inactivation by VIGS affected plant growth. In contrast, only clade 1-and clade 6-targeted plants showed modifications in outerstem tissue organization and secondary cell wall formation. In these plants, bast fibre number and structure were severely impacted, suggesting that the targeted genes may play an important role in the establishment of the fibre cell wall. Our results provide new fundamental information about cellulose biosynthesis in flax that should facilitate future plant improvement/ engineering.

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Functional analyses of cellulose synthase genes in flax (Linum usitatissimum) by virus‐induced gene silencing

Chantreau

Chabbert

Billiard

et al. 2015

Plant Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…These differential structural profiles may result in either a positive or a negative alteration of the protein, and contribute significantly to variations in cellulose production that might be related to plant fiber quality and biopolymer yield. The genera Corchorus and Gossypium displayed two types of tertiary structure that could be related to isoforms of the Ces genes described in these genera and, more recently also in soybean …”

Section: Biotechnological Advances In Agavementioning

confidence: 99%

“…22,143 These limitations make urgent to address studies toward the optimization of Agave crops for achieving higher yields and quality of cellulosic material that would possibly reduce the land extension and time needed for its cultivation. Molecular studies made in Gossypium spp., 144 Linum usitatissimum L., 145 and Nicotiana tabacum cv. Samsun NN 146 have shown that quality and yield of fiber can be improved by knowing the genetic and biochemical factors regulating plant biopolymer production.…”

Section: The Remarkable Mechanical Properties and Accessibility Ofmentioning

confidence: 99%

“…The genera Corchorus and Gossypium displayed two types of tertiary structure that could be related to isoforms of the Ces genes described in these genera and, more recently also in soybean. 145,147,153,161 Aiming at knowing if it was possible to find new applications based on primary aa sequences, we built dendrograms using 895 aa in the CESA like dominium-involved in the elongation of the glucan chain of cellulose-in 41 plant accessions, which grouped in close relation with their corresponding orders. The included orders were Fabales, Curcubitales, Malvales, Malpighiales, and Rosales, all of which have high economic importance as producers of fruits (Fabales and Curcubitales), seeds (Fabales), whole plants (Fabales), flowers (Rosales), and animal feed, and for decorative purposes.…”

Section: D Modeling Of Ces Egases and Susymentioning

confidence: 99%

See 1 more Smart Citation

Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest inAgaveL. for genetic improvement and biocatalysis

Tamayo‐Ordóñez

Ayil‐Gutiérrez

Tamayo-Ordóñez

et al. 2018

Biotechnology Progress

View full text Add to dashboard Cite

Several of the over 200 known species of Agave L. are currently used for production of distilled beverages and biopolymers. The plants live in a wide range of stressful environments as a result of their resistance to abiotic stress (drought, salinity, and extreme temperature) and pathogens, which gives the genus potential for germplasm conservation and biotechnological applications that may minimize economic losses as a result of the global climate change. However, the limited knowledge in the genus of genome structure and organization hampers development of potential improved biotechnological applications by means of genetic manipulation and biocatalysis. We reviewed Agave and plant sequences in the GenBank NCBI database for identifying genes with biotechnological potential for fermentation, bioenergy, fiber improvement, and in vivo plant biopolymer production. Three-dimensional modeling of enzyme structures in plant accessions revealed structural differences in sucrose 1-fructosyltransferase, fructan 1-fructosyltransferase, fructan exohydrolase (1-FEH), cellulose synthase (CES), and glucanases (EGases) with possible effects in fructan, sugar, and biopolymer production. Although the coding genes of FEH and enzymes involved in biopolymer production (CES, sucrose synthase, and EGases) remain unidentified in Agave L., our results could aid isolation of such genes in Agave. By comparing nucleotide and amino acid sequences in accessions of Agave and other plants, knowledge may be gained about transcriptional regulation and enzymatic activity factors. Future study is needed of biotechnological application of Agave genes for crop breeding aided by genetic engineering and biocatalysis.

show abstract

Expression analysis of cellulose synthase and main cytoskeletal protein genes in flax (Linum usitatissimum L.)

Galinousky

Padvitski

Bayer

et al. 2017

Cell Biology International

View full text Add to dashboard Cite

Fiber flax is an important source of natural fiber and a comprehensive model for the plant fiber biogenesis studies. Cellulose-synthase (CesA) and cytoskeletal genes are known to be important for the cell wall biogenesis in general and for the biogenesis of flax fibers in particular. Currently, knowledge about activity of these genes during the plant growth is limited. In this study, we have investigated flax fiber biogenesis by measuring expression of CesA and cytoskeletal genes at two stages of the flax development (seedlings and stems at the rapid growth stage) in several flax subspecies (elongatum, mediterraneum, crepitans). RT-qPCR has been used to quantify the expression of LusСesA1, LusСesA4, LusСesA7, LusСesA6, Actin, and α-Tubulin genes in plant samples. We report that CesA genes responsible for the secondary cell wall synthesis (LusCesA4, LusCesA7) have different expression pattern compared with CesA genes responsible for the primary cell wall synthesis (LusCesA1, LusCesA6): an average expression of LusCesA4 and LusCesA7 genes is relatively high in seedlings and further increases in stems at the rapid growth stage, whereas an average expression of LusCesA1 and LusCesA6 genes decreases. Interestingly, LusCesA1 is the only studied gene with different expression dynamics between the flax subspecies: its expression decreases by 5.2-10.7 folds in elongatum and mediterraneum but does not change in crepitans subspecies when the rapid growth stage and seedlings are compared. The expression of cytoskeleton genes (coding actin and α-tubulin) is relatively stable and significantly higher than the expression of cellulose-synthase genes in all the studied samples.

show abstract

Cellulose synthase genes that control the fiber formation of flax (Linum usitatissimum L.)

Cited by 9 publications

References 13 publications

Functional analyses of cellulose synthase genes in flax (Linum usitatissimum) by virus‐induced gene silencing

Functional analyses of cellulose synthase genes in flax (Linum usitatissimum) by virus‐induced gene silencing

Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest inAgaveL. for genetic improvement and biocatalysis

Expression analysis of cellulose synthase and main cytoskeletal protein genes in flax (Linum usitatissimum L.)

Contact Info

Product

Resources

About