To achieve broader range of the defensin antimicrobial activity, based on the sd2 gene sequence, the modified gene, sd2 mod, was constructed. Hybrid genes, sd2 -lic BM2, lic BM2-sd2 , lic BM2-sd2 mod, and sd2 mod-lic BM2, in which the wild-type and modified gene sequences were fused in frame with the reporter gene encoding thermostable lichenase, were constructed. Expression of the wild-type, modified, and hybrid genes was examined in the cells of pro-and eukaryotes. It was demonstrated that these genes were efficiently expressed in the cells of lower eukaryotes, the yeast. Inhibiting effect of the SD2 and SDmod proteins as the components of the hybrid proteins, SD2-LicBM2 and SD2mod-LicBM2, on the growth of the Fusarium culmorum hyphae was similar to that of the wild-type and modified proteins. It was shown that the presence of lichenase in the hybrid proteins facilitated selection and analysis of the hybrid proteins expression in transgenic organisms.
The article discusses the results of a four-year study (2016-2019) of breeding lines of spring soft wheat of the selection of Federal Agricultural Research Center of the North-East named N.V. Rudnitsky in the soil and climatic conditions of the central and southern parts of the Volga-Vyatka region. The promising lines C-65, C-103, H-154 were selected as they exceeded the regionalized standard variety Simbirtsit in average yields (3.41...3.56 t/ha; the increase is 0.04...0.19 t/ha), and in terms of potential yield (5.61…6.93 t / ha; the increase is 0.48...1.80 t/ha). According to the long-term average indicators of the crop structure, the selection lines П-57, P-63, С-65 and С-103 were attributed to the sources of valuable properties. Genotypes P-63, H-154 showed higher values and stability of gluten formation in grain relative to the variety Simbirtsit (22.8-24.1%; the increase to the standard is 3.1 - 4.4%). Using the variation coefficient, the degree of variability of economically useful traits (productive bushiness, plant height, ear length, number of grains per ear, grain weight per ear, 1000 grain mass, yield, gluten content in grain) was determined in different ecological environments. It has been established that the variability values of individual characteristics of productivity in one variety are not always consistent with each other and have genotypic conditionality. Yield stability can be closely related to the level of variation of individual elements of its structure. Genotypes that were more stable in yield indicated high variability of productive bushiness and low variability of plant height, grain weight per spike, and mass of 1000 grains. It has been shown that from practical point of view the most valuable genotypes were the ones with steadily high values of the spike length and the number of spike grains, as having a high adaptive response to cultivation conditions.Conflict of interest: the authors stated that there was no conflict of interest.
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