No abstract

The objective of this paper was to analyze the impact of the transition period on illicit drug use and its health consequences in Belarus, the Russian Federation and Ukraine. Available data were compiled to illustrate the trends and policy responses since 1991, when the countries had become politically independent and implemented radical changes in their social systems, which is particularly prominent in the Russian Federation and Ukraine. The transition period was associated with a rapid and dramatic increase in the supply of drugs combined with an increased demand, which was influenced by a range of social, economic and psychological factors. There was a sharp increase in negative public health consequences too. Inadequate policy responses might have had serious implications for economic and human development in the countries with a total population of more than 200 million people. The role of research to support policy responses is also discussed.

We studied the mechanisms of regenerative (wound healing) effects of songorine associated with functional activation of mesenchymal progenitor cells. The key role of FGF receptors on these progenitor cells in the stimulation of realization of their growth potential under the effect of the alkaloid was demonstrated. Under in vitro conditions, the antibodies to FGF receptor abolished the songorine-induced increase in the number of fibroblast colony-forming units in bone marrow cell culture. The intensity of differentiation of mesenchymal precursors remained unchanged.

Signal pathways of realization of growth potential of mesenchymal progenitor cells related to transcription factor NF-κB were studied in vitro. NF-κB was found to participate in the proliferation and differentiation of progenitor elements that can be blocked by its specific inhibitor oridonin. NF-κB inhibitor aurothiomalate had no effect on the functions of fibroblastic CFU.

Specific JNK and p53 inhibitors stimulated the formation of fibroblast colonies (CFU-F) and clusters (ClFU-F) and increased proliferative activity of mesenchymal progenitor cells. No effects of inhibitors of JNK and p53 on differentiation of progenitor elements were revealed.