Mechanisms of Regeneratory Effects of Baikal Aconite Diterpene Alkaloids

Involvement of cAMP- and IKK-2-Dependent Signal Pathways in the Growth Capacity of Mesenchymal Progenitor Cells under the Influence of Basic Fibroblast Growth Factor

“…This agent produced a stimulatory effect on proliferation of stromal precursor cells. Mitotic activity of mesenchymal progenitor cells was shown to decrease after treatment with a specifi c blocker of the IKK-2 kinase-inhibitor complex.The concept of protection and/or modifi cation of the functional response in survived cells [1] after medicinal treatment is sometimes inconsistent (e.g., during degenerative diseases) [1,13]. Therefore, the development of original drugs with a principally new mechanism of action is an urgent problem.…”

Mechanisms of Napelline Action Stimulating the Regeneration of Hemopoietic Tissue in Cytostatic Myelosuppression

“…2, b). However, given the data [12] on the stimulating effect of alkaloids from Aconitum baicalense on the differentiation of mesenchymal progenitors, it can be assumed that the identifi ed phenomenon might be the result of the accelerated maturation of above parental elements. The observed changes obviously determined accelerated restoration of hemopoietic tissue by the most rapid HIM recovery [6,7], which manifestations included enhanced CSA release by adherent myelocaryocytes.…”

“…In this, the mechanism of accelerated regeneration of hemopoietic tissue is the activation of hemopoietic progenitor cells apparently due to the direct action of alcaloid [12] and to increased feeder capacity of HIM stromal compartment. At the same time, the revealed pronounced effects of napelline on the functioning of fi broblast precursors comprising true (multipotent) stem cells in addition to the committed progenitor elements [10,11,13] indicates that the development of hemostimulants and also formulations for regenerative medicine based on napelling is promising.…”

“…Previously, we identifi ed that diterpene alkaloids stimulate mesenchymal progenitor cells and have marked wound-healing effect [12]. In addition, at the Institute of Pharmacology, Siberian Division of the Russian Academy of Medical Sciences, it was shown on the model of cytostatic myelosuppresion that the mixture of these substances extracted from Aconitum baicalense exerted signifi cant regenerative effects on blood system associated with the activation of bone marrow progenitor cells.…”

“…In addition, at the Institute of Pharmacology, Siberian Division of the Russian Academy of Medical Sciences, it was shown on the model of cytostatic myelosuppresion that the mixture of these substances extracted from Aconitum baicalense exerted signifi cant regenerative effects on blood system associated with the activation of bone marrow progenitor cells. At the same time, it was revealed that the expression of the effect on stem cells varied between diterpene alkaloids of different structure, among other factors, due to specifi city of their actions on the local regulatory mechanisms [12].…”