“…The in vitro working concentrations of inhibitors were estimated to be most effective (30 and 0.02 μM, respectively). Myelokaryocytes were cultured in the medium containing 80% DMEM medium, 20% FBS, 280 mg/liter L-glutamine, and 50 mg/liter gentamicin (Sigma) at 37 o C, 5% CO 2 , and absolute humidity for 7 days [10,12]. Proliferative activity of CFU-F was studied by the method of cellular self-destruction with hydroxyurea (Sigma) after 24-h preincubation of myelokaryocytes with inhibitors [2,10].…”