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Листовые болезни яровой пшеницы-бурая ржавчина (возбудитель-Puccinia triticina), желтая пятнистость (пиренофороз) (Pyrenophora tritici-repentis) и темно-бурая пятнистость (Сochliobolus sativus = Bipolaris sorokiniana)-относятся к группе распространенных и потенциально опасных болезней в западноазиатских регионах России и Северном Казахстане. Для обоснования стратегий генетической защиты пшеницы необходимы популяционные исследования фитопатогенов. Цель работыхарактеристика структуры популяций возбудителей бурой ржавчины и желтой пятнистости яровой пшеницы по признакам вирулентности и оценка распространенности возбудителя темно-бурой пятнистости в западноазиатских регионах Российской Федерации и Северном Казахстане в 2017 г. Источником инфекционного материала служили пораженные бурой ржавчиной и пятнистостями листья образцов яровой пше ницы, собранные в Челябинской и Омской областях и Северном Казахстане. Анализ вирулентности 109 изолятов P. triticina на 20 линиях-дифференциаторах показал, что все изученные монопустульные изоляты были авирулентны к ТсLr24. Изоляты, вирулентные к ТсLr19, выявлены только в челябинской популяции. Частоты вирулентных изолятов к ТсLr2a, ТсLr2b, ТсLr2c, ТсLr11, ТсLr15, ТсLr16, ТсLr20 и ТсLr26 были выше в омской и североказахстанской популяциях, а к ТсLr9-в челябинской. При использовании 20 ТсLr-ли ний определено 27 фенотипов вирулентности P.

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Brown rust is the main disease of wheat soft varieties in the Rostov region. The majority of wheat varieties (more than 87%) developed in the FSBSI “Agricultural Research Center “Donskoy” show resistance to this causative agent. In order to obtain a complete immunological characteristics of the developed varieties, there were carried out the researches to identify the resis­tance genes (Lr) in the early and adult stages of plant growing in cooperation with the Mycology and Phytopathology Laboratory of the FSBSI ARIZR. There were studied 37 varieties of winter soft wheat on the infectious fields of the south and northwest of Russia, as well as by the methods of a phytopathological test (to three isolates of the fungus with virulence to TcLr9, TcLr19, TcLr26 and the Zernograd pathogen population) and by the PCR analysis using 10 molecular markers Lr1, Lr3, Lr9, Lr10, Lr19, Lr20, Lr24, Lr26, Lr34 and Lr37. DNA was isolated by a micro-method according to K. Edwards, the PCR was carried out in a C-1000 amplifier (Bio Rad, US). The study established that there were no universal varieties among the studied ones which were resistant to all three clones and the Zergograd population. The varieties “Polina”, “Volnitsa” and “Zernogradka 11” showed resistance to the three clones, but in the phase of sprouting they show susceptibility to the pathogen population. According to the results of PCR analysis, the resistance genes Lr9, Lr19, Lr24, Lr26 were also not found in the varieties. 29 out of 37 studied varieties contained the adult resistance gene Lr34, and 20 varieties had the inefficient gene Lr3, which neither apart nor together could protect the plants from brown rust in the field. This indicates that the tolerant varieties carry additional non-identified Lr-genes. In a number of varieties there have been established 2 resistance genes. The variety “Kipchak” susceptible in the field contained the Lr1 gene, which lost its effectiveness. The differences in the damage degree of some varieties in the conditions of the Rostov region and St. Peters­burg indicate a difference in the North Caucasian and northwestern populations by virulence to the causative agent of brown rust.

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Background. One of the promising sources for enrichment of the common wheat (Triticum aestivum L.) gene pool with new alleles is synthetic hexaploid wheat (SHW), or allopolyploids from crossing tetraploid wheats (2n = 4x = 28, BBAA) with accessions of Aegilops tauschii Coss. (2n = 2x = 14, DD), and subsequent doubling of the chromosome number in the hybrids. Objectives of the study were to evaluate the SHW accessions from the VIR collection for resistance to Puccinia triticina Erikss. populations collected in Russia; genotype the accessions; and summarize information from the published sources concerning the resistance of the studied accessions to other harmful diseases and pests.Materials and methods. Resistance of 36 SHW accessions from the VIR collection to the populations of P. triticina was assessed in the laboratory and in the field, under artificial infection pressure, using the techniques developed by the Institute of Plant Protection. A phytopathological test and PCR markers were used to identify the Lr genes.Results and conclusion. The SHW accessions were characterized according to their resistance to the Russian populations of the wheat leaf rust pathogen. The sources of resistance in the phase of emergence and in adult plants were identified. The phytopathological test isolated three accessions with Lr23; the PCR marker of Lr21=Lr40 was found in 11 accessions, Lr39=Lr41 in 19, and Lr22a in 3. At the same time, k-65496, k-65515 and k-65517 had si multaneously Lr21=Lr40 and Lr39=Lr41, while k-65497, k-65503 and k-65508 had Lr22a and Lr39=Lr41. The analysis of published data showed that many of the studied SHW accessions were also resistant to other harmful diseases and insect pests, so they are of interest for further studying and possible use in domestic breeding.

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