We studied the effect of interleukin-1beta on functional activity of lymphoid structures in the gastrointestinal tract of rats with various behavioral parameters during stress of simultaneous immobilization and electrocutaneous stimulation. Morphofunctional characteristics of lymphoid tissue were estimated by studying elimination of intraperitoneally injected Chinese ink particles into the mesenteric lymph nodes and wall of the jejunum. Intraperitoneal injection of interleukin-1beta (5 mug/kg, 10(8) U/mg) was accompanied by accumulation of Chinese ink in the mesenteric lymph nodes of unstressed passive and active rats. The observed changes reflect an immunostimulatory effect of this cytokine. Acute stress was followed by an increase in the number of ink particles in the mesenteric lymph nodes and wall of the jejunum in behaviorally active rats. Under these conditions, the number of ink particles was elevated only in the mesenteric lymph nodes of passive specimens. As differentiated from passive animals, pretreatment of active rats with interleukin-1beta before acute stress was followed by the increased elimination of Chinese ink (antigenic material) from the abdominal cavity to the lymph nodes and through the wall of the jejunum. These data illustrate specific features of immune mechanisms for the stress response in mammals with various behavioral characteristics.
The study revealed that the severity and duration of response of the lymphoid formations in the walls of the small intestine to acute emotional stress depends on the individual organism's resistance to the stress exposure. Despite the fungible nature of the response of lymphoid structures in the stressors, their reaction in the group of stress-resistant rats was less pronounced, and recovery times are shorter compared to the stress-prone animals.
We studied the effect of acute emotional stress on functional status of lymphoid epithelial structures of the jejunum in rats with different behavioral activity. Morphological and functional characteristics of lymphoid tissue were assessed using morphometric, histological and electron microscopic methods. In behaviorally active and passive rats, reduction in villus height, area of the epithelium and lymphoid tissue of the jejunum was recorded on the third day after acute emotional impact. At that, the largest number of destructively modified lymphoid cells was identified by comparison with the other time points. Moreover, destruction of the apical part of the jejunal villi was observed on days 3 and 7 after stress exposure. Recovery of the lymphoepithelial structures of the jejunum after acute stress exposure was detected on day 14.
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