The strain Rhodococcus ruber P25 utilizes 4 chlorobiphenyl (4CB) and 4 chlorobenzoic acid (4CBA) as sole carbon and energy sources. 4CB degradation by washed cells of strain P25 was accompanied by transient formation of 4CBA, followed by its utilization and release of equimolar amounts of chloride ions into the medium. The strain R. ruber P25 possessed active enzyme systems providing 4CBA degradation via the stages of formation of intermediates, para hydroxybenzoate (PHBA) and protocatechuic acid (PCA), to compounds of the basic metabolism. The involvement of protocatechuate 4,5 dioxygenase in 4CBA degra dation by rhodococci was revealed. It was established that the initial stage of 4CBA degradation (dehaloge nation) in the strain R. ruber P25 was controlled by the fcbA and fcbB genes encoding 4 CBA CoA ligase and 4 CBA CoA dehalogenase, respectively. The genes encoding 4CBA dehalogenase components have not been previously detected and characterized in bacteria of the genus Rhodococcus.
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