Degradation of 4-chlorobiphenyl and 4-chlorobenzoic acid by the strain Rhodococcus ruber P25

Плотникова, Е. Г.; Solyanikova, Inna P.; Егорова, Д. О.; Шумкова, Е. С.; Golovleva, L. A.

doi:10.1134/s0026261712020117

Cited by 22 publications

(6 citation statements)

References 32 publications

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“…Strains P25 and B51 were found to degrade chlorinated biphenyls efficiently from mono to hexachlorobiphenyls which includes planar congeners too. It was recognized that these strains are able to utilize a variety of chlorobiphenyls as medium for growth without requiring supplementary carbon source and accumulated non/less toxic byproducts in the environment (Rybkina et al 2003; Egorova et al 2011; Plotnikova et al 2012). In recent past, R. ruber P25 and Microbacterium sp.…”

Section: Bioaugmentation Of Aerobic Organismsmentioning

confidence: 99%

Advances and perspective in bioremediation of polychlorinated biphenyl-contaminated soils

Sharma

Gautam

Nanekar

et al. 2017

Environ Sci Pollut Res

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In recent years, microbial degradation and bioremediation approaches of polychlorinated biphenyls (PCBs) have been studied extensively considering their toxicity, carcinogenicity and persistency potential in the environment. In this direction, different catabolic enzymes have been identified and reported for biodegradation of different PCB congeners along with optimization of biological processes. A genome analysis of PCB-degrading bacteria has led in an improved understanding of their metabolic potential and adaptation to stressful conditions. However, many stones in this area are left unturned. For example, the role and diversity of uncultivable microbes in PCB degradation are still not fully understood. Improved knowledge and understanding on this front will open up new avenues for improved bioremediation technologies which will bring economic, environmental and societal benefits. This article highlights on recent advances in bioremediation of PCBs in soil. It is demonstrated that bioremediation is the most effective and innovative technology which includes biostimulation, bioaugmentation, phytoremediation and rhizoremediation and acts as a model solution for pollution abatement. More recently, transgenic plants and genetically modified microorganisms have proved to be revolutionary in the bioremediation of PCBs. Additionally, other important aspects such as pretreatment using chemical/physical agents for enhanced biodegradation are also addressed. Efforts have been made to identify challenges, research gaps and necessary approaches which in future, can be harnessed for successful use of bioremediation under field conditions. Emphases have been given on the quality/efficiency of bioremediation technology and its related cost which determines its ultimate acceptability.

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Section: Bioaugmentation Of Aerobic Organismsmentioning

confidence: 99%

Advances and perspective in bioremediation of polychlorinated biphenyl-contaminated soils

Sharma

Gautam

Nanekar

et al. 2017

Environ Sci Pollut Res

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“…The presence of both orthoand metacleavage pathways in Sphe3 was also validated through proteomic analysis [29]. Although the occurrence of meta-cleavage of PCA has also been demonstrated in Arthrobacter keyseri 12B [30] as well as in other Actinobacteria [31], there is no study on the kinetic characterization of PCA 4,5-dioxygenase in this phylum. The functional and/or kinetic studies of enzymes belonging to the largely uncharacterized Type II extradiol dioxygenase clade of the PCAD-Memo superfamily will allow comparisons among these enzymes, thus shedding more light on this Type in the dioxygenase superfamily.…”

Section: Introductionmentioning

confidence: 97%

Characterization of Protocatechuate 4,5-Dioxygenase from Pseudarthrobacter phenanthrenivorans Sphe3 and In Situ Reaction Monitoring in the NMR Tube

Tsagogiannis

Vandera

Primikyri

et al. 2021

IJMS

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The current study aims at the functional and kinetic characterization of protocatechuate (PCA) 4,5-dioxygenase (PcaA) from Pseudarthrobacter phenanthrenivorans Sphe3. This is the first single subunit Type II dioxygenase characterized in Actinobacteria. RT-PCR analysis demonstrated that pcaA and the adjacent putative genes implicated in the PCA meta-cleavage pathway comprise a single transcriptional unit. The recombinant PcaA is highly specific for PCA and exhibits Michaelis–Menten kinetics with Km and Vmax values of 21 ± 1.6 μM and 44.8 ± 4.0 U × mg−1, respectively, in pH 9.5 and at 20 °C. PcaA also converted gallate from a broad range of substrates tested. The enzymatic reaction products were identified and characterized, for the first time, through in situ biotransformation monitoring inside an NMR tube. The PCA reaction product demonstrated a keto-enol tautomerization, whereas the gallate reaction product was present only in the keto form. Moreover, the transcriptional levels of pcaA and pcaR (gene encoding a LysR-type regulator of the pathway) were also determined, showing an induction when cells were grown on PCA and phenanthrene. Studying key enzymes in biodegradation pathways is significant for bioremediation and for efficient biocatalysts development.

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“…PCBs undergoes dehalogenation to lower chlorinated biphenyl and further to chlorobenzoic acid metabolite. These chlorobiphenyl is further converted into hydroxybenzoic acid and dihydroxybenzoic acid ( Zhai et al, 2010 ; Plotnikova et al, 2012 ). Bedard et al (1987) , have reported chlorinated benzoic acid and chlorinated acetophenone intermediate metabolites from oxidation of PCB congeners.…”

Section: Resultsmentioning

confidence: 99%

PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil

Sandhu

Paul²,

Proćków³

et al. 2022

Front. Microbiol.

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Polychlorinated biphenyls (PCBs) are persistent organic pollutants widely distributed in the environment and possess deleterious health effects. The main objective of the study was to obtain bacterial isolates from PCB-contaminated soil for enhanced biodegradation of PCB-77. Selective enrichment resulted in the isolation of 33 strains of PCB-contaminated soil nearby Bhilai steel plant, Chhattisgarh, India. Based on the prominent growth using biphenyl as the sole carbon source and the confirmation of its degradation by GC-MS/MS analysis, four isolates were selected for further study. The isolates identified by 16S rRNA gene sequencing were Pseudomonas aeruginosa MAPB-2, Pseudomonas plecoglossicida MAPB-6, Brucella anthropi MAPB-9, and Priestia megaterium MAPB-27. The isolate MAPB-9 showed a degradation of 66.15% biphenyl, while MAPB-2, MAPB-6, and MAPB-27 showed a degradation of 62.06, 57.02, and 56.55%, respectively in 48 h. Additionally, the degradation ability of these strains was enhanced with addition of co-metabolite glucose (0.2%) in the culture medium. Addition of glucose showed 100% degradation of biphenyl by MAPB-9, in 48 h, while MAPB-6, MAPB-2, and MAPB-27 showed 97.1, 67.5, and 53.3% degradation, respectively as analyzed by GC-MS/MS. Furthermore, in the presence of inducer, PCB-77 was found to be 59.89, 30.49, 27.19, and 4.43% degraded by MAPB-6, MAPB-9, MAPB-2, and MAPB-27, respectively in 7 d. The production of biosurfactants that aid in biodegradation process were observed in all the isolates. This was confirmed by ATR-FTIR analysis that showed the presence of major functional groups (CH2, CH3, CH, = CH2, C–O–C, C-O) of the biosurfactant. The biosurfactants were further identified by HPTLC and GC-MS/MS analysis. Present study is the first to report PCB-77 degradation potential of Pseudomonas aeruginosa, B. anthropi, Pseudomonas plecoglossicida, and Priestia megaterium. Similarly, this is the first report on Pseudomonas plecoglossicida and Priestia megaterium for PCB biodegradation. Our results suggest that the above isolates can be used for the biodegradation of biphenyl and PCB-77 in PCB-contaminated soil.

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Degradation of 4-chlorobiphenyl and 4-chlorobenzoic acid by the strain Rhodococcus ruber P25

Cited by 22 publications

References 32 publications

Advances and perspective in bioremediation of polychlorinated biphenyl-contaminated soils

Advances and perspective in bioremediation of polychlorinated biphenyl-contaminated soils

Characterization of Protocatechuate 4,5-Dioxygenase from Pseudarthrobacter phenanthrenivorans Sphe3 and In Situ Reaction Monitoring in the NMR Tube

PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil

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