Brucella strains isolated from mouselike rodents in the northern foothills of the Large Caucasus are phenotypically similar to Brucella suis. On the basis of several differential characteristics, these strains are considered to be a new biotype of B. suis.During the last 20 years, strains of Brucella have been recovered from some species of mice and mouselike rodents (Apodemus agrarius Pall., Mus musculus L., Apodemus sylvaticus L., Microtus arvalis Pall., Micromys minutus Pall.) in the northern foothills of the Large Caucasus. Investigations of these strains by several workers (1, 2, 6) produced contrasting opinions concerning the taxonomic position of these isolates.In this paper we report the results of taxonomic studies of rodent strains of Brucella that were conducted as recommended by the Subcommittee on Taxonomy of the genus Brucella (3).A total of 66 Brucella strains isolated from mouselike rodents in S-form were selected for this study. International reference strains Brucelfa melitensis 16-MT (type strain) biotype 1, Brucella' abortus 544T biotype 1, Brucella suis 1330T biotype 1, B. suis Thomsen biotype 2, B. suis 686 biotype 3, and B. suis 40 biotype 4 were used for comparison. These strains were obtained from the Laboratory of Brucellosis, Gamaleya Institute of Epidemiology and Microbiology, Moscow.The morphological and staining characteristics of the rodent strains were typical of Brucella. Cultures grew aerobically and did not produce hydrogen sulfide. The rodent isolates studied, as well as the other Brucella strains, hydrolyzed urea and contained neuraminidase with an activity of 135 HE. All strains grew in the presence of thionine at standard concentrations of 1:25,000, 1:50,000, and 1:lOo,O00. Growth was inhibited by basic fuchsin (1 50,000, 1:100,0oO). The rodent strains were lysed by brucellaphages Wb (Weybridge) and Bkz (Berkeley) at 1 and 104 routine test dilutions and by phages Tb (Tbilisi) and Fi (Firenze) only at 104 routine test dilutions, which is characteristic of B. suis. These strains were agglutinated with monospecific anti-B. melitensis serum at a titer of 1:320, but not with anti-B. abortus serum. These results differentiate the rodent strains from the previously described biotypes of B. suis. Oxidative metabolism tests (5) showed that the rodent strains oxidized L-arginine, DL-ornithine, DL-citrulline, and L-lysine. Oxidation of these substrates is characteristic of B. suis. However, unlike B. suis, the rodent strains also oxidized L-alanine, L-asparagine , and L-glutamic acid. Standard concentrations of pyronin B (1:800,000), safranin T (1:500,000), gentian violet (1:500,000), and crystal violet (1:2,000,000) suppressed growth of the rodent strains but did not inhibit growth of the reference strains. This feature differentiates the rodent strains. The lipopolysaccharides from the rodent strains were similar to those of the reference strain of B. suis in chemical composition, antigenic structure, and toxicity for white mice.An investigation of the protein spectra of Brucella st...
Given are the results of assessment of epidemiological and epizootiological situation on brucellosis in the Russian Federation in 2015. It is characterized as challenging. Compared to 2014 (368 cases, intensive index -0.26), recorded is 5.1 % increase in the number of people infected with brucellosis (393 cases, intensive index -0.27). The highest incidence rates among the population are registered in the NorthCaucasian, Southern and Siberian Federal Districts, where the main source of infection is cattle and small ru minants. Revealed is a significant rise in brucellosis incidence in the Stavropol region too. External epidemiological risks, associated with the intensification of cooperation in the supply of livestock products from the Mediterranean countries, Middle East, and South America, which are endemic as regards cattle brucellosis, will remain pressing. Given the sustained adverse epidemiological and epizootic situation, taking into account the internal and external risks of infection with brucellosis, brucellosis incidence among the population is predicted to be within the range of 390-420 cases.
Displayed is the analysis of epizootic and epidemic situation on brucellosis in the Russian Federation in 2012. Demonstrated is the fact that epizootic condition still remains tense. It specifies the character of epidemic manifestations. For 2013 leveling of the brucellosis incidence rate among the population of the Russian Federation is forecasted.
Carried out is the analysis and assessment of epizootiologic and epidemiologic situation on brucellosis in the Russian Federation over the year 2013. It is demonstrated that on the grounds of tense epizootic situation there is a tendency to reduction in brucellosis morbidity rates among the population. Incidence rates in the North-Caucasian and Southern Federal districts remain high due to B. melitensis spp. brucellosis agent circulation. However, realization of a complex of brucellosis prophylactic measures in the territory of the Republic of Dagestan has resulted in a considerable reduction of a number of primary cases in the region. In addition, forecasted is stabilization of brucellosis morbidity rates among the population and farming animals in 2014, being estimated at 320-370 cases a year.
Материалы и методы Штаммы бруцелл, питательные среды, реактивы. В работе использовали 59 культур бруцелл шести видов (B. melitensis, B. abortus, B. suis, B. canis, B. ovis, B. neotomae) из коллекции микроорганизмов Ставропольского противочумного института, в том числе 11 референсных штаммов: B. abortus 19 BA (вакцинный), B. abortus 544 (1 биовар, референсный), B. abortus 1552, B. abortus 870 (6 биовар, референсный), B. abortus C-68 (9 биовар, референсный), B. abortus 63-75 (7 биовар, референсный), B. abortus B-3196 (5 биовар, референсный), B. abortus Tulya (3 биовар, референсный), B. abortus C-548 (6 биовар), B. abortus C-549 (6 биовар), B. abortus C-550 (3 биовар), B. abortus 19 (1 биовар), B. abortus C-497, B. abortus C-551 (3 биовар), B. abortus С-499 (3 биовар), B. melitensis 16-M (1 биовар, референсный), B. melitensis 63/9 (2 биовар), B. melitensis 548 (1 биовар), B. melitensis C-554 (3 биовар), B. melitensis Rev-1 (1 биовар, вакцинный), B. melitensis C-555 (3 биовар), B. melitensis C-556 (1 биовар), B. meliten-sis C-557 (1 биовар), B. melitensis C-558 (3 биовар), B. melitensis C-559 (1 биовар), B. melitensis Ether 706 (3 биовар, референсный), B. melitensis C-560, B. melitensis C-561, B. melitensis C-562, (3 биовар), B. ovis 707, B. suis 61, B. suis 513, B. suis 1330 (1 биовар, референсный), B. suis Thomsen (2 биовар, референсный), B. suis 40, B. suis 686 (3 биовар, референсный), B. suis 484, B. suis 539, B. suis 513, B. suis S-705, B. suis 68-86, B. suis 68 (4 биовар), B. suis 323 (4 биовар), B. suis 324, B. canis 6/66, B. canis 1066 (1 биовар), B. canis Н-966, B. neotomae 65/198, B. neotomae 65/196, B. neotomae 325 (1 биовар), B. neotomae 66/2 (1 биовар), B. neotomae 5K33, B. suis И-72, B. suis 2-1/20-2, B. suis И-299, B. suis И-200, B. ovis 712, B. ovis С-440 (№ 6), B. ovis 722; воду ультрачистую (тип I по ASTM) (система Millipore, США), спирт этиловый 96 % (ГОСТ Р 51723-2001), кислоту муравьиную, ~ 98 % (Sigma-Aldrich, США), ацетонитрил (степень чистоты «для ВЭЖХ-МС») (Sigma-Aldrich, США), α-циано-4-гидроксикоричную кислоту (степень чистоты для масс-спектрометрии) (Sigma-Aldrich, США), трифторуксусную кислоту, > 99 %
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