Given are the results of assessment of epidemiological and epizootiological situation on brucellosis in the Russian Federation in 2015. It is characterized as challenging. Compared to 2014 (368 cases, intensive index -0.26), recorded is 5.1 % increase in the number of people infected with brucellosis (393 cases, intensive index -0.27). The highest incidence rates among the population are registered in the NorthCaucasian, Southern and Siberian Federal Districts, where the main source of infection is cattle and small ru minants. Revealed is a significant rise in brucellosis incidence in the Stavropol region too. External epidemiological risks, associated with the intensification of cooperation in the supply of livestock products from the Mediterranean countries, Middle East, and South America, which are endemic as regards cattle brucellosis, will remain pressing. Given the sustained adverse epidemiological and epizootic situation, taking into account the internal and external risks of infection with brucellosis, brucellosis incidence among the population is predicted to be within the range of 390-420 cases.
Carried out is the analysis and assessment of epizootiologic and epidemiologic situation on brucellosis in the Russian Federation over the year 2013. It is demonstrated that on the grounds of tense epizootic situation there is a tendency to reduction in brucellosis morbidity rates among the population. Incidence rates in the North-Caucasian and Southern Federal districts remain high due to B. melitensis spp. brucellosis agent circulation. However, realization of a complex of brucellosis prophylactic measures in the territory of the Republic of Dagestan has resulted in a considerable reduction of a number of primary cases in the region. In addition, forecasted is stabilization of brucellosis morbidity rates among the population and farming animals in 2014, being estimated at 320-370 cases a year.
Материалы и методы Штаммы бруцелл, питательные среды, реактивы. В работе использовали 59 культур бруцелл шести видов (B. melitensis, B. abortus, B. suis, B. canis, B. ovis, B. neotomae) из коллекции микроорганизмов Ставропольского противочумного института, в том числе 11 референсных штаммов: B. abortus 19 BA (вакцинный), B. abortus 544 (1 биовар, референсный), B. abortus 1552, B. abortus 870 (6 биовар, референсный), B. abortus C-68 (9 биовар, референсный), B. abortus 63-75 (7 биовар, референсный), B. abortus B-3196 (5 биовар, референсный), B. abortus Tulya (3 биовар, референсный), B. abortus C-548 (6 биовар), B. abortus C-549 (6 биовар), B. abortus C-550 (3 биовар), B. abortus 19 (1 биовар), B. abortus C-497, B. abortus C-551 (3 биовар), B. abortus С-499 (3 биовар), B. melitensis 16-M (1 биовар, референсный), B. melitensis 63/9 (2 биовар), B. melitensis 548 (1 биовар), B. melitensis C-554 (3 биовар), B. melitensis Rev-1 (1 биовар, вакцинный), B. melitensis C-555 (3 биовар), B. melitensis C-556 (1 биовар), B. meliten-sis C-557 (1 биовар), B. melitensis C-558 (3 биовар), B. melitensis C-559 (1 биовар), B. melitensis Ether 706 (3 биовар, референсный), B. melitensis C-560, B. melitensis C-561, B. melitensis C-562, (3 биовар), B. ovis 707, B. suis 61, B. suis 513, B. suis 1330 (1 биовар, референсный), B. suis Thomsen (2 биовар, референсный), B. suis 40, B. suis 686 (3 биовар, референсный), B. suis 484, B. suis 539, B. suis 513, B. suis S-705, B. suis 68-86, B. suis 68 (4 биовар), B. suis 323 (4 биовар), B. suis 324, B. canis 6/66, B. canis 1066 (1 биовар), B. canis Н-966, B. neotomae 65/198, B. neotomae 65/196, B. neotomae 325 (1 биовар), B. neotomae 66/2 (1 биовар), B. neotomae 5K33, B. suis И-72, B. suis 2-1/20-2, B. suis И-299, B. suis И-200, B. ovis 712, B. ovis С-440 (№ 6), B. ovis 722; воду ультрачистую (тип I по ASTM) (система Millipore, США), спирт этиловый 96 % (ГОСТ Р 51723-2001), кислоту муравьиную, ~ 98 % (Sigma-Aldrich, США), ацетонитрил (степень чистоты «для ВЭЖХ-МС») (Sigma-Aldrich, США), α-циано-4-гидроксикоричную кислоту (степень чистоты для масс-спектрометрии) (Sigma-Aldrich, США), трифторуксусную кислоту, > 99 %
Represented is the analysis and assessment of epidemiological and epizootiological situation on brucellosis in the Russian Federation in 2014. Human morbidity rates in 2014 exceed the indexes of 2013 by 7.7 %. Negative trend in the livestock animal incidence (cattle and small ruminants), as well as in the numbers of registered index potentially hazardous territories, as regards brucellosis, remains. The highest brucellosis morbidity rates are in the North-Caucasian, Southern and Siberian Federal Districts. The quantity of brucellosis cases among the population has increased in Central and Far-Eastern Federal Districts. Put forward is the prognosis on the human brucellosis morbidity rates for 2015.
Aim. Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar. Materials and methods. Vaccine strains: Brucella melitensis Rev-1 and Brucella abortus 19BA. Protein profiling in linear mode on Microflex «Bruker Daltonics» MALDI-TOF mass-spectrometer. Results. A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used. Conclusion. Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.
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