A nanostructured mineral food supplement with a particle size of 60.0-120.0 nm was manufactured from phosphorite by ultrasonic dispersion. It was found that intragastric administration of nanostructures phosphorite to mice is relatively safe: clinical signs of intoxication appeared after a single administration of the preparation only at a dose of 90 mg/kg; a dose of 150 mg/kg caused death of 8% of mice, in which injuries of organs of the gastrointestinal tract were observed. When the preparation was administered subcutaneously, intramuscularly, or intraperitoneally, small phosphorite conglomerates and inflammation of the surrounding tissues and organs were observed at the injection site. Death of 25% of animals was observed in the group of mice which received intraperitoneal injections of nanophosphorite at a dose of 200 mg/kg.
The article shows the results of researches of the natural zeolites influence on potato yield increase. It was found that the treated seed tubers and plants during the growing season with activated zeolite separately and in combination contributed to an increase in crop yield of 3.0-3.6%, when treated with an ultrafine analogue-by 4.3-12. 8% to the background. The marketability of potatoes in the variants where activated zeolite was used varied from 90.9 to 92.9%. When using ultrafine zeolite yield of commercial tubers was 92.9-97.1%. Ultrafine zeolite of particle sizes 80.0-350.0 nm was produced by ultrasonic action on activated zeolite (0.25-40.0 microns) at a frequency of 15.0 Hz (±10%). It was found that high biological activity of ultradispersed zeolite in comparison with actvated analog is caused by highly developed surface. In the process of dispersion of agglomerates and aggregates (associations of primary particles and crystallites) of actvated zeolite, a more developed specific surface of ultrafine zeolite is formed, which increases the number of active sites. Consequently, the processes of interaction between particles, including with a biological object, increase.
Local natural zeolite-containing rocks use efficiency in agriculture of the Republic of Tatarstan was studied in the research. The research was conducted in the field on leached black soil applying mechanoactivated zeolites and in greenhouses on grey forest soil using nanostructured zeolite. Field experiments identified the soil deacidification of 0.4 pHsol, increase of assimilable phosphates of the 1st and 2nd groups (Ca-P1 и Ca-P2) – of 5.0 and 6.6 mg per 100 g of soil. Moreover, the research considered the increasing the mobile phosphorus available to plants – of 8.0 and 10.0 mg/kg and exchange potassium of 5.0 and 6.6 mg/kg according to the doses of zeolite of 10 and 15 t/ha. Improving the activity of microflora in rhizosphere and growth acceleration of microbial biomass to 15.5 mg per 100 g*h were observed. The average annual grain yield gain was 0.3 and 0.5 t/ha, and the payback of 1 ton of zeolite by additional harvest 0.11 and 0.09 t/ha in grain units respectively. Improvement in the reaction of the soil environment was stated in the vegetative conditions on gray forest soil using nanostructured zeolite in doses of 0.1 and 1.0 t/ha, a positive shift in pHsol, was 0.97 and 0.9 units respectively. Under the influence of mechanically activated celite at a dose of 15 t/ha, soil acidity decreased by 0.99 pHsol units. The use of nanostructured zeolite contributed to an increase of the reserves of ammonia nitrogen in the soil – by 0.8 and 2.4 mg/kg, mobile phosphorus – by 6.0 and 33.0 mg/kg, and exchangeable potassium – by 3.0 and 5.5 mg/kg respectively at doses of 0.1 and 1.0 t/ha respectively. The growth of microbial biomass was up to 24.0 mg/100 g*h. Additional grain gains were obtained: under the influence of mechanically activated zeolite at a dose of 15 t/ha – 8.6 g/vessel and nanostructured analog at a dose of 0.1 t/ha – 7.3 g/vessel, which were comparable at the consumption of the latter 150 times less.
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