Mesenchymal stem cells from the adipose tissue of patients with postoperative hernias produce excessive amounts of collagen III, which shifts the balance between type III and type I collagens. The proposed technique of pretransplantation preparation allows in vitro stimulation collagen formation processes with predominant activation of collagen I synthesis and normalization of proportion between different collagen types. Abdominal wall repair with polypropylene surgical mesh in combination with autotransplantation of mesenchymal stem cells reduced the collagen III to collagen I ratio due to activation of collagen I synthesis and suppression of collagen III production, which had a positive effect on the structure of in vivo formed connective tissue.
Summery. Introduction. The research devoted to the study of pathogenetic, immunological, and biophysical aspects of the tissue regeneration process with wound defects of various etiologies is gaining in popularity in the "world" of medical science, at present this problem remains topical [1, 2, 6, 8, 9]
Purpose of the study. A comparative analysis of the dynamics of morphological manifestations of tissue regeneration occurring when recombinant interferon α2b, is used in the healing of an acute experimental wound is carried out.
Materials and methods. Depending on the nature of the wound treatment, the experimental animals were divided into two groups: 1) a comparison group in which the edges of the wound were struck with a 0.9% NaCl solution; 2) the main group, where a similar edge of the wound defect was cut off with recombinant interferon α2b at a dose of 1000 ME / 1ml diluted with 0.9% NaCl solution. The rats were removed from the experiment on the 4th, 8th and 20th day of the study, while a full-thickness skin flap was taken in the area of the scar (defect), which was examined by light microscopy. At the same time, the intensity of formation of the granulation tissue, its "maturation", and also in the margins and on the bottom of the wound was estimated in the absolute values, the cellular populations realizing the process of inflammation were determined.
As a result of the study, the character of the complex effect of recombinant interferon α2b was established, which included reduction of the negative effect of secondary alteration, differentiation of the intravascular immune response, formation of conditions for natural regeneration with activation of angiogenesis and synthesis of a valuable scar, decrease in propensity to keloidosis.
Conclusions. The obtained results testify to the morphological validity of the use of recombinant leukocyte interferon α2β as a stimulant of the inflammation phase, as against the background of the use of this substance, its ability to enhance phagocytosis was noted.
В кратком обзоре дано представление о препаратах растворимых факторов тромбоцитов (РФТ) человека и их медицинском применении. Отмечено, что препараты РФТ уже широко используются для лечения длительно заживающих ран кожи и слизистых, а также при травмах и заболеваниях опорно-двигательного аппарата. Существуют области медицины, в которых активно изучаются условия для успешного местного применения препаратов РФТ: клеточная терапия, пластическая хирургия, офтальмология, кожные болезни, косметология, офтальмология, акушерство и гинекология, неврология. Сделан вывод о целесообразности дальнейшего медицинского изучения показаний для эффективного применения препаратов РФТ.
A brief review provides the data on the preparations of human soluble platelet factors (SPF) and their medical use. It is noted that SPF preparations are widely used for the treatment of long-term healing wounds of the skin and mucous membranes, as well as for injuries and diseases of the musculoskeletal system. There are the areas of medicine, where the conditions for successful topical use of SPF are being actively studied. These are the cell therapy, plastic surgery, ophthalmology, skin diseases, cosmetology, obstetrics and gynecology, neurology. The conclusion is made about the expediency of further medical study of the indications for the effective use of SPF preparations.
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