Raman and Fourier Transform Infrared (FT-IR) spectroscopy was used for investigation of structural differences of bacterial celluloses (BC), obtained by cultivation native and immobilized cells of Gluconacetobacter sucrofermentans during static and dynamic regimes of cultivation on a molasses media. It was found that the Raman and FT-IR spectra could characterized the groups of the cellulose molecules. The culturing bacterial cellulose in the presence of results in an increase of crystalline and it increased during cultivated on a molasses media with the addition of 1.5% ethanol—75.62%. The degree of BC crystallinity increased during dynamic regime of cultivation is higher than under static regime one. The maximal BC content was observed when 0.5% ascorbic acid was added to the cultivation medium with molasses and native cells. It was found, the degree of BC crystallinity during static regime cultivation on a molasses medium with ethanol, increased significantly to 73.5%, and during dynamic regime—75.6%. So, in this study, the changes of the bacterial cellulose conformation of were revealed during bacterial cultivation in a medium containing molasses in various cultivation modes.
The infrared spectroscopy was applied on kernels of the following maize inbred lines with significant traits: ZPPL 186, ZPPL 225 and M1-3-3 Sdms, with the aim to determine structural properties of organic compounds and their unstable state. The set hypothesis was that it was necessary to observe the existence of numerous and different spectral bands, not studied so far, occurring in various patterns (bands of low intensity, single or grouped) and to explain the nature and the dynamics of their formation. Such spectral bands were observed in the wave number range of 400-2925 cm -1 and are caused by the different types of vibration movements (valence and deformation vibrations) of organic compounds: alkenes, aromatic compounds, alcohols, ethers, carboxylic acids, esters, amines, amides, alkanes, nitro compounds, ketones, aldehydes, alkynes, nitriles and phenols. In this way, it is possible to establish not only the structure of organic compounds of kernels of observed maize inbred lines, but also it is possible to point out to their unstable, conformational and functional properties. The importance of intensity, shape and kinetics of spectral bands, expressing unstable processes in biological systems and bioactive organic molecules, has been studied and emphasised for the first time.
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