Supramolecular structure formation of poly(3-hydroxybutyrate) ultrathin fibers prepared by electrospinning and the influence of low concentrations of silicon andTiO 2 nanoparticles on the structure, physicomechanical and sorption properties, and resistance to thermal destruction and thermoand photo-oxidative destruction of non-woven fibrous material based on these fibers were studied. It was found that nanoparticles promoted the formation of thinner fibers with enhanced physicomechanical parameters and a structure that was more resistant to thermal and thermo-and photo-oxidative destruction and had a positive effect on the growth dynamics of mesenchymal stem cells.Tissue engineering utilizes various polymeric constructs prepared by one of the most promising methods, i.e., electrospinning (ES) of fibrous nanocomposites from biopolymers [1-3]. The method allows fibrous scaffolds with large surface-to-volume ratios to be produced. This enables free migration and proliferation of cells into the three-dimensional matrix and; therefore, excellent integration of the material into living tissues [4].Polyoxyalkanoates (POA), the most scrutinized of which is poly(3-hydroxybutyrate) (PHB), are special biopolymers [5,6]. PHB is prepared by a biotechnology route, possesses promising properties such as a high level of biocompatibility [7][8][9], and can undergo biodegradation in living tissues without forming toxic substances [10][11][12]. Therefore, PHB is used in the development of medical implements for surgery, dentistry, cardiac surgery, orthopedics, and other areas [13,14].The diameter of a monofilament and its molecular and supramolecular structure affect its physicomechanical and diffusion properties, the kinetics of oxidative and photo-oxidative reactions, and the biodegradation of fibrous non-woven materials [7]. The diameter of fibers produced by ES depends on parameters such as the concentration of the substance in the starting solution, solvent evaporation rate, potential, distance from the needle to the spun layer, electrical conductivity, viscosity, temperature, etc.[4]. Therefore, the production of a finished fiber with a definite morphology continues to be a critical problem.However, the diameter of PHB ultrathin fibers is practically unaffected by changing the ES parameters (electrical conductivity, throughput, spinning solution viscosity) [15,16]. Therefore, the structure and properties of non-woven PHB materials remain practically unchanged.The creation of modern bioresorbable fibrous materials for use as matrices for targeted drug delivery, scaffolds for growing living cells, artificial ligaments, etc. requires a method for varying the PHB fiber structural parameters in order to construct materials with the desired array of properties.
The composite material based on reinforcement of polyamide filaments enclosed by a nonwoven matrix of nanoscaled bioresorbable poly(3-hydroxybutyrate) fibers was developed for application as an artificial ligament implant. The aim of this study was to investigate biodegradability and biocompatibility of the developed implant, as well as its stress-strain properties. The study results show the polyamide core of the implant has stress-strain properties comparable with a natural ligament. Simultaneously, the polyhydroxybutyrate external layer provides high biocompatibility and bioresorbability of the developed implant. The material has proven to be effective under in vivo tests with experimental rats as a ligament replacement for damaged Achilles tendons. Due to cell attachment and growth on the fibrous matrix during 5 weeks postsurgery, regenerated connective tissue was formed substituting for the polymeric implant, which confirmed its efficiency in contrast to the polyamide filament implant with a much longer resorption time. The results obtained indicate application prospects of polyamide-polyhydroxybutyrate implants for reconstructive surgery. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2708-2713, 2018.
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