The levels of 17 cytokines in the follicular fluid were measured by multiplex proteome analysis at the peak of stimulated ovulation during the in vitro fertilization cycle. In patients with ineffective folliculogenesis, the concentrations of IL-2, IL-4, IL-7, and granulocytic CSF in the follicular fluid were significantly lower than in women with greater mean number of collected oocytes. It was shown that multicomponent cytokine deficit was associated with lower production of high quality oocytes and lower efficiency of fertilization of the resultant oocytes in vitro. The absence of pregnancy after in vitro fertilization cycle is associated with lower levels of IL-2, IL-4, IL-7, granulocytic CSF, and macrophagic inflammatory protein 1b in combination with elevated contents of IL-8 and IL-13. The results attest to an important role of cytokines in the regulation of oogenesis and in preparation of the endometrium to implantation of the embryo.
Резюме. С помощью метода ранговой корреляции по Спирману оценивали направленность и вы-раженность взаимосвязи между показателями морфологических нарушений сперматозоидов с уров-нем цитокинов в семенной плазме мужчин, находящихся в длительном бесплодном браке. Корре-ляционный анализ показал наличие значимой положительной связи между частотой встречаемости морфологически аномальных сперматозоидов с уровнем IL-1β, MIP-1β, G-CSF и отрицательной с содержанием IL-5, IL-10 в семенной плазме. В целом полученные результаты отражают участие ци-токинов в регуляции мужской фертильности. Modes of interaction between sperMatozoid abnorMalities and cytokines in seMinal plasMa froM infertile Malesabstract. Spearman rank correlation analysis was applied to assess some interrelations between morphological abnormalities of spermatozoids in males with long-term infertility, and cytokine levels in their seminal plasma. Such statistical analysis has revealed a significant positive correlation between prevalence of morphologically abnormal spermatozoids and the levels of IL-1β, MIP-1β, G-CSF, along with negative relationship with IL-5 and IL-10 contents in seminal fluid. The data are, generally, demonstrating participation of cytokines in regulation of male fertility.
The study involved 107 women aged 20 to 52 years with chronic recurrent cystitis (57 of them with leukoplakia) developing under conditions of a pathogenic and/or opportunistic infection. All patients underwent a conventional clinical and instrumental examination. Biopsy samples for culture-based and microscopic examination were taken from the area of the Pawlik’s trigone of the vagina and the Lieutaud’s trigone of the urinary bladder, in case of leukoplakia — from the field of visually healthy tissue. The carried out cytomorphological analysis and assessment of pathological processes in the adjacent tissues suggest that the infection from the vagina penetrates through the intersynaptic clefts, interstitial cell nests, the blood and lymphatic microcirculatory system into the bladder, causing cystitis inherent changes — from initial morphological forms to rough ones, up to leukoplakia. A complex of clinical, functional and pathomorphological changes that fit into the framework of the chronic cystovaginitis concept was identified.
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