A method of atomic force microscopy-based fishing (AFM fishing) has been developed for protein detection in the analyte solution using a chip with an immobilized aptamer. This method is based on the biospecific fishing of a target protein from a bulk solution onto the small AFM chip area with the immobilized aptamer to this protein used as the molecular probe. Such aptamer-based approach allows to increase an AFM image contrast compared to the antibody-based approach. Mass spectrometry analysis used after the biospecific fishing to identify the target protein on the AFM chip has proved complex formation. Use of the AFM chip with the immobilized aptamer avoids interference of the antibody and target protein peaks in a mass spectrum.
Цель работы - анализ ключевых постгеномных технологий, ожидаемых в рамках реализации приоритета научно-технологического развития, определённого пунктом 20в «Переход к персонализированной медицине, высокотехнологичному здравоохранению и технологиям сохранения здоровья, в том числе за счет рационального применения лекарственных препаратов (прежде всего антибактериальных)» СНТР Российской Федерации». Результаты. Обозначены наиболее перспективные направления дальнейших исследований. Мировой уровень развития постгеномных технологий (ПГТ) позволяет перейти от этапов исследований и разработок к внедрению в медицинскую практику. На сегодняшний день к основным направлениям успешного практического применения ПГТ в России и за рубежом относят биофармацевтику, включая разработку методов генного редактирования для лечения онкологических и орфанных заболеваний, развитие методов молекулярного профилирования для персонализированной медицины и питания, увеличения активного возраста человека. Заключение. Быстрое развитие высокопроизводительных постгеномных технологий и вычислительных систем позволило беспрепятственно исследовать биологические системы. Индивидуальные и интегративные постгеномные профили полезны для мониторинга состояния здоровья человека, оказания превентивных мероприятий и выбора эффективной лекарственной терапии. The aim of this study was to analyze key expected postgenomic technologies as a part of priority in scientific and technological development defined in item 20 of the Strategy for Scientific and Technological Development of the Russian Federation, «Transition to personalized medicine, high-tech health care and health saving technologies through the rational use of medicines (primarily antibacterial)». Results. The most promising areas of further research were identified. The international level of postgenomic technologies (PGT) allows to move from research and development to implementation in medical practice. Presently, industrial biotechnology, biopharmaceuticals, including development of gene editing methods for treatment of oncological and orphan diseases and molecular profiling methods for individualized medicine, nutrition, and prolonging the active life are considered the main directions for successful practical use of PGT in Russia and other countries. Conclusion. Rapid development of high-performance postgenomic technologies and computer systems has expedited studying biological systems. Individual and integrative postgenomic profiles are useful for monitoring the state of human health, taking preventive measures, and selecting effective drug therapy.
We performed the analysis of the markets and sectors of the economy, the development of which is ensured by the implementation of the priority 20v «the transition to personalized medicine, high-tech health care and health protection technologies» in the field of post-genomic technologies, including genome editing and molecular profiling, based on the analysis of big data, considering the key Russian and foreign strategic and forecast documents, forecasts of large corporations and consulting agencies, news and scientific open Russian and foreign resources. the analysis showed the active development of the market for post-genomic technologies in Russia. Market segments demonstrated high growth rates, following in line with global trends. State institutions had adopted a number of programs aimed at supporting and developing the sector of practical implementation of post-genomic technologies. the policy of the Government of the Russian Federation, aimed at importing foreign drugs and medical products, created a full cycle of domestic production of targeted drugs for personalized therapy of malignant tumors.
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